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Both strains are highly resistant to their own antibiotics. Independent expression of the<jats:italic>S. lydicus<\/jats:italic>and<jats:italic>S. spectabilis rpoB<\/jats:italic>and<jats:italic>rpoC<\/jats:italic>genes, encoding the \u03b2- and \u03b2\u2032-subunits of RNA polymerase, respectively, in<jats:italic>S. albus<\/jats:italic>showed that resistance is mediated by<jats:italic>rpoB<\/jats:italic>, with no effect of<jats:italic>rpoC<\/jats:italic>. Within the \u03b2-subunit, resistance was confined to an amino acid region harboring the \u201crif region.\u201d Comparison of the \u03b2-subunit amino acid sequences of this region from the producer strains and those of other streptomycetes and site-directed mutagenesis of specific differential residues located in it (L485 and D486 in<jats:italic>S. lydicus<\/jats:italic>and N474 and S475 in<jats:italic>S. spectabilis<\/jats:italic>) showed their involvement in streptolydigin and streptovaricin resistance. Other amino acids located close to the \u201cStl pocket\u201d in the<jats:italic>S. lydicus<\/jats:italic>\u03b2-subunit (L555, F593, and M594) were also found to exert influence on streptolydigin resistance.<\/jats:p>","DOI":"10.1128\/aac.01406-09","type":"journal-article","created":{"date-parts":[[2010,2,23]],"date-time":"2010-02-23T03:24:40Z","timestamp":1266895480000},"page":"1684-1692","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Involvement of the Beta Subunit of RNA Polymerase in Resistance to Streptolydigin and Streptovaricin in the Producer Organisms<i>Streptomyces lydicus<\/i>and<i>Streptomyces spectabilis<\/i>"],"prefix":"10.1128","volume":"54","author":[{"given":"Marina","family":"Sa\u0301nchez-Hidalgo","sequence":"first","affiliation":[{"name":"Departamento de Biologi\u0301a Funcional e Instituto Universitario de Oncologi\u0301a del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz Elena","family":"Nu\u0301n\u0303ez","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Funcional e Instituto Universitario de Oncologi\u0301a del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Me\u0301ndez","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Funcional e Instituto Universitario de Oncologi\u0301a del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 A.","family":"Salas","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Funcional e Instituto Universitario de Oncologi\u0301a del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.05.017"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti770"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.8.2276"},{"key":"e_1_3_2_6_2","first-page":"2883","volume":"130","year":"1984","unstructured":"Blanco, M. 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