{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:13:49Z","timestamp":1785428029446,"version":"3.56.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["TTU 08.806"],"award-info":[{"award-number":["TTU 08.806"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"name":"German Research Council","award":["390838134"],"award-info":[{"award-number":["390838134"]}]},{"name":"European Society for Paediatric Gastroenterology, Hepatology, and Nutrition","award":["ESPGHAN Research Grant"],"award-info":[{"award-number":["ESPGHAN Research Grant"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,5,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Staphylococcus capitis<\/jats:italic>\n                      NRCS-A is a major cause of neonatal sepsis worldwide and exhibits resistance to multiple antibiotics. We assessed the prevalence and mechanisms of daptomycin resistance (DAP-R) in bloodstream isolates from a German neonatal intensive care unit. Ten of 11 NRCS-A isolates (91%) were resistant to daptomycin, and 18% displayed decreased susceptibility to vancomycin. Genomic analysis revealed diverse polymorphisms in genes associated with DAP-R in\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus,<\/jats:italic>\n                      but no single amino acid variant explained resistance. Phospholipid composition remained unchanged, whereas isolates displayed increased cell surface charge and cell wall thickening. Consistently, BODIPY-labeled daptomycin binding was reduced and more diffusely distributed in DAP-R NRCS-A with weaker septal enrichment. In serial passaging experiments,\n                      <jats:italic toggle=\"yes\">S. capitis<\/jats:italic>\n                      acquired DAP-R more rapidly and robustly than\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      or\n                      <jats:italic toggle=\"yes\">S. epidermidis<\/jats:italic>\n                      under subinhibitory concentrations of either daptomycin or vancomycin. Resequencing after\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      DAP-R evolution in\n                      <jats:italic toggle=\"yes\">S. capitis<\/jats:italic>\n                      identified newly acquired mutations in cell envelope-associated genes, including\n                      <jats:italic toggle=\"yes\">walK<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">mprF<\/jats:italic>\n                      . These results indicate that\n                      <jats:italic toggle=\"yes\">S. capitis<\/jats:italic>\n                      NRCS-A rapidly evolves resistance via polygenic, cell-envelope-driven mechanisms distinct from those in\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      . The high prevalence and adaptive capacity of DAP-R in neonatal isolates raise concern for therapeutic failure in neonatal intensive care.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01414-25","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T14:17:51Z","timestamp":1774361871000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Polygenic, cell-envelope adaptations drive high-frequency daptomycin resistance in\n                    <i>Staphylococcus capitis<\/i>\n                    NRCS-A from neonatal sepsis and NEC"],"prefix":"10.1128","volume":"70","author":[{"given":"Lara","family":"Kr\u00e4nkel","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/03esvmb28","id-type":"ROR","asserted-by":"publisher"}],"name":"Pediatric Surgery and Urology, University Children\u00b4s Hospital T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna","family":"Hauser","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Slavetinsky","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Zinser","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/03esvmb28","id-type":"ROR","asserted-by":"publisher"}],"name":"Pediatric Surgery and Urology, University Children\u00b4s Hospital T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Schmidt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mulugeta","family":"Nega","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03a1kwz48","id-type":"ROR","asserted-by":"publisher"}],"name":"Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine T\u00fcbingen (IMIT), University of T\u00fcbingen","place":["T\u00fcbingen, Germany."]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M. A.","family":"Elsherbini","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Schneider","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01xnwqx93","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Pharmaceutical Microbiology, University Hospital Bonn","place":["Bonn, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gille","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03esvmb28","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Neonatology, University Children's Hospital T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Schaller","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Fuchs","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03esvmb28","id-type":"ROR","asserted-by":"publisher"}],"name":"Pediatric Surgery and Urology, University Children\u00b4s Hospital T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3209-8626","authenticated-orcid":true,"given":"Andreas","family":"Peschel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]},{"name":"Cluster of Excellence EXC 2124 Controlling Microbes to Fight Infections","place":["T\u00fcbingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/03a1kwz48","id-type":"ROR","asserted-by":"publisher"}],"name":"Infection Biology, Interfaculty Institute of Microbiology and Infection Medicine T\u00fcbingen (IMIT), University of T\u00fcbingen","place":["T\u00fcbingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/028s4q594","id-type":"ROR","asserted-by":"publisher"}],"name":"German Center for Infection Research (DZIF), partner site T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5576-5906","authenticated-orcid":true,"given":"Christoph","family":"Slavetinsky","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ht1tz09","id-type":"ROR","asserted-by":"publisher"}],"name":"Interfaculty Institute of Microbiology and Infection Medicine, Cellular and Molecular Microbiology Division, University of Tu\u0308bingen","place":["Tu\u0308bingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/03esvmb28","id-type":"ROR","asserted-by":"publisher"}],"name":"Pediatric Surgery and Urology, University Children\u00b4s Hospital T\u00fcbingen","place":["T\u00fcbingen, Germany"]},{"name":"Cluster of Excellence EXC 2124 Controlling Microbes to Fight Infections","place":["T\u00fcbingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/028s4q594","id-type":"ROR","asserted-by":"publisher"}],"name":"German Center for Infection Research (DZIF), partner site T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-019-03106-z"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2015.02.038"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.694093"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pedneo.2017.06.001"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2014-306213"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000002976"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2020-320217"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.119"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2005-0552"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2022-058813"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031548"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01991"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0676-2"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.09.008"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv217"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13030254"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10020168"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1542\/neo.23-10-e738"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.4.532"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.31.4.625"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00413a009"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14060617"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-016-0130-8"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-39486-8"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.1c00316"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.93267"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15257-1"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2014.05.014"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00023-18"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2019.08.021"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02705"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06819.x"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1453233"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198366"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1101\/2023.04.11.536441"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00563-23"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv225"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07576.x"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01926-18"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.9.1067"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29717-3"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.194"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66376"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01990-23"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000660"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3345"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.13.8405"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01659-18"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00039-06"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028316"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr195"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02182-12"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.1035574"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1812066116"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002359"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03599.x"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja808971c"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.173836"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01531-18"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00492-17"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001106"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001340"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a026997"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.5-14.2003"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00160-15"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.5491045"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01414-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01414-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T13:01:56Z","timestamp":1778072516000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.01414-25"}},"subtitle":[],"editor":[{"given":"Benjamin P.","family":"Howden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,5,6]]},"references-count":68,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5,6]]}},"alternative-id":["10.1128\/aac.01414-25"],"URL":"https:\/\/doi.org\/10.1128\/aac.01414-25","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,6]]},"assertion":[{"value":"2025-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01414-25"}}