{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T11:24:56Z","timestamp":1761391496606},"reference-count":32,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The main goal of this work is to clarify the predictive value of known genetic markers of\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            resistance to penicillin, tetracycline, and fluoroquinolones. The correlation between the presence of certain genetic markers and susceptibility of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates to penicillin, tetracycline, and fluoroquinolones has been analyzed by means of statistical methods. Susceptibility testing with penicillin, tetracycline, and fluoroquinolones was performed by the agar dilution method.\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            genomic DNA was isolated. The presence of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-1<\/jats:sub>\n            and\n            <jats:italic>tet<\/jats:italic>\n            (\n            <jats:italic>M<\/jats:italic>\n            ) genes was analyzed by PCR. A novel method of polymorphism discovery based on a minisequencing reaction followed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry was applied for the analysis of chromosomal\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            genes involved in antimicrobial resistance development. Clinical\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates (\n            <jats:italic>n<\/jats:italic>\n            = 464) were collected. Susceptibility levels to penicillin, tetracycline, and fluoroquinolones were found to be 25.9%, 35.9%, and 54.1%, respectively. Among the 19\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates with penicillin MICs of \u22654 \u03bcg\/ml, the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-1<\/jats:sub>\n            gene was detected in 12. The Tet(M) determinant was found in 4 of 12\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates with tetracycline MICs of \u226516 \u03bcg\/ml. The chromosomal genetic markers of penicillin and tetracycline resistance were detected especially in isolates with penicillin MICs of 0.25 to 2.0 \u03bcg\/ml and tetracycline MICs of 0.5 to 4 \u03bcg\/ml. Mutations in GyrA and ParC were found in 208 of 211 quinolone-resistant\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates. This work is the first representative molecular research of the\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            population in Russia. Information about the prevalence of antibiotic resistance mechanisms and the positive predictive value of certain genetic determinants is given. The positive predictive values of the analyzed genetic markers were found to be different for fluoroquinolones (90.3%), penicillin (91.1%), and tetracycline (81.9%).\n          <\/jats:p>","DOI":"10.1128\/aac.01420-07","type":"journal-article","created":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:46:09Z","timestamp":1207010769000},"page":"2175-2182","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Relation between Genetic Markers of Drug Resistance and Susceptibility Profile of Clinical\n            <i>Neisseria gonorrhoeae<\/i>\n            Strains"],"prefix":"10.1128","volume":"52","author":[{"given":"Elena N.","family":"Ilina","sequence":"first","affiliation":[{"name":"Research Institute of Physico-Chemical Medicine, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir A.","family":"Vereshchagin","sequence":"additional","affiliation":[{"name":"Research Institute of Physico-Chemical Medicine, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra D.","family":"Borovskaya","sequence":"additional","affiliation":[{"name":"Research Institute of Physico-Chemical Medicine, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja V.","family":"Malakhova","sequence":"additional","affiliation":[{"name":"Research Institute of Physico-Chemical Medicine, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergei V.","family":"Sidorenko","sequence":"additional","affiliation":[{"name":"Central Research Institute of Dermatology and Venerology, Moscow, Russian Federation"},{"name":"National Research Centre for Antibiotics, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazar C.","family":"Al-Khafaji","sequence":"additional","affiliation":[{"name":"Central Research Institute of Dermatology and Venerology, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna A.","family":"Kubanova","sequence":"additional","affiliation":[{"name":"Central Research Institute of Dermatology and Venerology, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vadim M.","family":"Govorun","sequence":"additional","affiliation":[{"name":"Research Institute of Physico-Chemical Medicine, Moscow, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00664.x"},{"key":"e_1_3_2_4_2","unstructured":"Performance standards for antimicrobial susceptibility testing; 15th informational supplement. 2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.ijantimicag.2004.01.001","volume":"23","year":"2004","unstructured":"Cousin, S., Jr., M. 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Malakhova, V. A. Vereshchagin, V. M. Govorun, V. I. Sergienko, M. M. Zubkov, M. M. Vasil'ev, and A. A. Kubanova. 2003. Molecular typing of N. gonorrhoeae strains prevalent in the Russian Federation. Bull. Exp. Biol. Med.136:179-182.","journal-title":"Bull. Exp. Biol. Med."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.04.003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.13.4123-4128.1997"},{"key":"e_1_3_2_18_2","unstructured":"About the statement of the protocol of conducting patients with gonococcal infection. 2003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.5.664"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1128\/AAC.46.9.2811-2820.2002","volume":"46","year":"2002","unstructured":"Olesky, M., M. Hobbs, and R. A. Nicholas. 2002. 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