{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:47:12Z","timestamp":1791010032732,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Amphotericin B (AMB) is the most widely used polyene antifungal drug for the treatment of systemic fungal infections, including invasive aspergillosis. It has been our aim to understand the molecular targets of AMB in\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    by genomic and proteomic approaches. In transcriptomic analysis, a total of 295 genes were found to be differentially expressed (165 upregulated and 130 downregulated), including many involving the ergosterol pathway, cell stress proteins, cell wall proteins, transport proteins, and hypothetical proteins. Proteomic profiles of\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    alone or\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    treated with AMB showed differential expression levels for 85 proteins (76 upregulated and 9 downregulated). Forty-eight of them were identified with high confidence and belonged to the above-mentioned categories. Differential expression levels for Rho-GDP dissociation inhibitor (Rho-GDI), secretory-pathway GDI, clathrin, Sec 31 (a subunit of the exocyst complex), and RAB GTPase Ypt51 in response to an antifungal drug are reported here for the first time and may represent a specific response of\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    to AMB. The expression of some of these genes was validated by real-time reverse transcription-PCR. The AMB responsive genes\/proteins observed to be differentially expressed in\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    may be further explored for novel drug development.\n                  <\/jats:p>","DOI":"10.1128\/aac.01431-07","type":"journal-article","created":{"date-parts":[[2008,10,6]],"date-time":"2008-10-06T20:46:15Z","timestamp":1223325975000},"page":"4220-4227","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":94,"title":["Proteomic and Transcriptomic Analysis of\n                    <i>Aspergillus fumigatus<\/i>\n                    on Exposure to Amphotericin B"],"prefix":"10.1128","volume":"52","author":[{"given":"Poonam","family":"Gautam","sequence":"first","affiliation":[{"name":"Molecular Biochemistry and Diagnostics Division, Institute of Genomics and Integrative Biology, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jata","family":"Shankar","sequence":"additional","affiliation":[{"name":"Molecular Biochemistry and Diagnostics Division, Institute of Genomics and Integrative Biology, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taruna","family":"Madan","sequence":"additional","affiliation":[{"name":"Molecular Biochemistry and Diagnostics Division, Institute of Genomics and Integrative Biology, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Sirdeshmukh","sequence":"additional","affiliation":[{"name":"Proteomics Research Facility, Centre for Cellular and Molecular Biology, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Curam Sreenivasacharlu","family":"Sundaram","sequence":"additional","affiliation":[{"name":"Proteomics Research Facility, Centre for Cellular and Molecular Biology, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wasudev Namdeo","family":"Gade","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, University of Pune, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seemi Farhat","family":"Basir","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Jamia Millia Islamia, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puranam Usha","family":"Sarma","sequence":"additional","affiliation":[{"name":"Molecular Biochemistry and Diagnostics Division, Institute of Genomics and Integrative Biology, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306291200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.2.453-460.2006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.3946-3951.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.5.1255-1265.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.39.1.9.17"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/138945005774912717"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.615-624.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1042\/bj3460423","volume":"346","year":"2000","unstructured":"Chow, L. 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