{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T14:27:29Z","timestamp":1777645649587,"version":"3.51.4"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia cepacia<\/jats:named-content>\n            complex (BCC) bacteria are opportunistic pathogens that can cause severe disease in cystic fibrosis (CF) patients and other immunocompromised individuals and are typically multidrug resistant. Here we observed that unlike other BCC species, most environmental and clinical\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia vietnamiensis<\/jats:named-content>\n            isolates were intrinsically susceptible to aminoglycosides but not to cationic antimicrobial peptides or polymyxin B. Furthermore, strains acquired aminoglycoside resistance during chronic CF infection, a phenomenon that could be induced under tobramycin or azithromycin pressure\n            <jats:italic>in vitro<\/jats:italic>\n            . In comparing susceptible and resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. vietnamiensis<\/jats:named-content>\n            isolates, no gross differences in lipopolysaccharide structure were observed, all had lipid A-associated 4-amino-4-\n            <jats:italic>deoxy<\/jats:italic>\n            -\n            <jats:sc>l<\/jats:sc>\n            -arabinose residues, and all were resistant to the permeabilizing effects of aminoglycosides, a measure of drug entry via self-promoted uptake. However, susceptible isolates accumulated 5 to 6 times more gentamicin than a resistant isolate, and aminoglycoside susceptibility increased in the presence of an efflux pump inhibitor.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. vietnamiensis<\/jats:named-content>\n            is therefore unusual among BCC bacteria in its susceptibility to aminoglycosides and capacity to acquire resistance. Aminoglycoside resistance appears to be due to decreased cellular accumulation as a result of active efflux.\n          <\/jats:p>","DOI":"10.1128\/aac.01434-10","type":"journal-article","created":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T10:20:09Z","timestamp":1297765209000},"page":"2256-2264","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["<i>In Vitro<\/i>\n            Susceptibility of Burkholderia vietnamiensis to Aminoglycosides"],"prefix":"10.1128","volume":"55","author":[{"given":"Agatha N.","family":"Jassem","sequence":"first","affiliation":[{"name":"Departments of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"Centre for Understanding and Preventing Infection in Children, University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E. 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