{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:44:20Z","timestamp":1789429460654,"version":"build-2803163510"},"reference-count":89,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,5,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Phage therapy has (re)emerged as a serious possibility for combating multidrug-resistant bacterial infections, including those caused by vancomycin-resistant\n              <jats:italic>Enterococcus faecium<\/jats:italic>\n              strains. These opportunistic pathogens belong to a specific clonal complex 17, against which relatively few phages have been screened. We isolated a collection of 21 virulent phages growing on these vancomycin-resistant isolates. Each of these phages harbored a typical narrow plaquing host range, lysing at most 5 strains and covering together 10 strains of our panel of 14 clinical isolates. To enlarge the host spectrum of our phages, the Appelmans protocol was used. We mixed four out of our most complementary phages in a cocktail that we iteratively grew on eight naive strains from our panel, of which six were initially refractory to at least three of the combined phages. Fifteen successive passages permitted to significantly improve the lytic activity of the cocktail, from which phages with extended host ranges within the\n              <jats:italic>E. faecium<\/jats:italic>\n              species could be isolated. A single evolved phage able to kill up to 10 of the 14 initial\n              <jats:italic>E. faecium<\/jats:italic>\n              strains was obtained, and it barely infected nearby species. All evolved phages had acquired point mutations or a recombination event in the tail fiber genetic region, suggesting these genes might have driven phage evolution by contributing to their extended host spectra.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01439-23","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T12:37:17Z","timestamp":1712666237000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["The virtue of training: extending phage host spectra against vancomycin-resistant\n            <i>Enterococcus faecium<\/i>\n            strains using the Appelmans method"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8364-7234","authenticated-orcid":true,"given":"Julien","family":"Lossouarn","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Beurrier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Bouteau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Moncaut","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Sir Silmane","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u00efdi","family":"Portalier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Zouari","sequence":"additional","affiliation":[{"name":"CHU de Rennes, Service de Bact\u00e9riologie-Hygi\u00e8ne Hospitali\u00e8re et CNR de la R\u00e9sistance aux Antibiotiques (laboratoire associ\u00e9 \"Ent\u00e9rocoques\"), Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Cattoir","sequence":"additional","affiliation":[{"name":"CHU de Rennes, Service de Bact\u00e9riologie-Hygi\u00e8ne Hospitali\u00e8re et CNR de la R\u00e9sistance aux Antibiotiques (laboratoire associ\u00e9 \"Ent\u00e9rocoques\"), Rennes, France"},{"name":"Universit\u00e9 de Rennes, INSERM, UMR_S1230 BRM, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8446-9011","authenticated-orcid":true,"given":"Pascale","family":"Serror","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Agn\u00e8s","family":"Petit","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","first-page":"3","volume-title":"Enterococci: from commensals to leading causes of drug resistant infection. 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Enterococcal disease, epidemiology, and implications for treatment, p 47\u201372. In Gilmore MS, Clewell DB, Ike Y, Shankar N (ed), Enterococci: from commensals to leading causes of drug resistant infection. Massachusetts eye and ear infirmary. Boston, MA."},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2761"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2018.08.016"},{"key":"e_1_3_4_9_2","first-page":"89","volume-title":"Enterococci: from commensals to leading causes of drug resistant infection. Massachusetts eye and ear infirmary","author":"Kristich CJ","year":"2014","unstructured":"Kristich CJ, Rice LB, Arias CA. 2014. Enterococcal infection\u2014treatment and antibiotic resistance, p 89\u2013134. In Gilmore MS, Clewell DB, Ike Y, Shankar N (ed), Enterococci: from commensals to leading causes of drug resistant infection. Massachusetts eye and ear infirmary. 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