{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:47:09Z","timestamp":1791150429041,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A carbapenem-resistant\n            <jats:italic>Pseudomonas stutzeri<\/jats:italic>\n            strain isolated from a Dutch patient was analyzed in detail. This isolate produced a metallo-\u03b2-lactamase (MBL) whose gene, with 43.5% GC content, was cloned and expressed in\n            <jats:italic>Escherichia coli.<\/jats:italic>\n            \u03b2-Lactamase DIM-1 (for\n            <jats:italic>D<\/jats:italic>\n            utch\n            <jats:italic>im<\/jats:italic>\n            ipenemase) was weakly related to other Ambler class B \u03b2-lactamases, sharing &lt;52% amino acid identity with the most closely related MBL, GIM-1, and 45% identity with IMP-type MBLs. The \u03b2-Lactamase DIM-1 significantly hydrolyzed broad-spectrum cephalosporins and carbapenems and spared aztreonam. This MBL gene was embedded in a class 1 integron containing two other gene cassettes, encoding resistance to aminoglycosides and disinfectants, that was located on a 70-kb plasmid.\n          <\/jats:p>","DOI":"10.1128\/aac.01456-09","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T01:42:20Z","timestamp":1269308540000},"page":"2420-2424","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Characterization of DIM-1, an Integron-Encoded Metallo-\u03b2-Lactamase from a\n            <i>Pseudomonas stutzeri<\/i>\n            Clinical Isolate in the Netherlands"],"prefix":"10.1128","volume":"54","author":[{"given":"Laurent","family":"Poirel","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, Universite\u0301 Paris XI, Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose-Manuel","family":"Rodri\u0301guez-Marti\u0301nez","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, Universite\u0301 Paris XI, Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nashwan","family":"Al Naiemi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Vrije Universiteit Medical Center, Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette J.","family":"Debets-Ossenkopp","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Vrije Universiteit Medical Center, Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, Universite\u0301 Paris XI, Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1878-1886.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.10.3223-3227.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/514922"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4654-4661.2004"},{"key":"e_1_3_2_7_2","unstructured":"Performance standards for antimicrobial susceptibility testing. 2010"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.155"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.10.008"},{"key":"e_1_3_2_10_2","unstructured":"Doumith M. 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