{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:31:57Z","timestamp":1790602317394,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"French Ministry of Defense | Direction G\u00e9n\u00e9rale de l'Armement","award":["FastGeneI"],"award-info":[{"award-number":["FastGeneI"]}]},{"DOI":"10.13039\/100013281","name":"Joint Programming Initiative on Antimicrobial Resistance","doi-asserted-by":"crossref","award":["ANR-19-JAMR-0004"],"award-info":[{"award-number":["ANR-19-JAMR-0004"]}],"id":[{"id":"10.13039\/100013281","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Universit\u00e4t Freiburg | National Center of Competence in Research Bio-Inspired Materials, University of Fribourg"},{"DOI":"10.13039\/501100001711","name":"Swiss national science foundation","doi-asserted-by":"crossref","award":["FNS-407240_177381"],"award-info":[{"award-number":["FNS-407240_177381"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Laboratoire Europeen Associe; INSERM \"Emerging antibiotic resistance in Gram-negative bacteria\""}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,4,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Beta-lactamase-mediated degradation of beta-lactams is the most common mechanism of beta-lactam resistance in Gram-negative bacteria. Beta-lactamase-encoding genes can be transferred between closely related bacteria, but spontaneous inter-phylum transfers (between distantly related bacteria) have never been reported. Here, we describe an extended-spectrum beta-lactamase (ESBL)-encoding gene (\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>MUN-1<\/jats:sub>\n                      ) shared between the Pseudomonadota and Bacteroidota phyla. An\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      strain was isolated from a patient in M\u00fcnster (Germany). Its genome was sequenced. The ESBL-encoding gene (named\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>MUN-1<\/jats:sub>\n                      ) was cloned, and the corresponding enzyme was characterized. The distribution of the gene among bacteria was investigated using the RefSeq Genomes database. The frequency and relative abundance of its closest homolog in the global microbial gene catalog (GMGC) were analyzed. The\n                      <jats:italic>E. coli<\/jats:italic>\n                      strain exhibited two distinct morphotypes. Each morphotype possessed two chromosomal copies of the\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>MUN-1<\/jats:sub>\n                      gene, with one morphotype having two additional copies located on a phage-plasmid p0111. Each copy was located within a 7.6-kb genomic island associated with mobility.\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>MUN-1<\/jats:sub>\n                      encoded for an extended-spectrum Ambler subclass A2 beta-lactamase with 43.0% amino acid identity to TLA-1.\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>MUN-1<\/jats:sub>\n                      was found in species among the Bacteroidales order and in\n                      <jats:italic>Sutterella wadsworthensis<\/jats:italic>\n                      (Pseudomonadota). Its closest homolog in GMGC was detected frequently in human fecal samples. This is, to our knowledge, the first reported instance of inter-phylum transfer of an ESBL-encoding gene, between the Bacteroidota and Pseudomonadota phyla. Although the gene was frequently detected in the human gut, inter-phylum transfer was rare, indicating that inter-phylum barriers are effective in impeding the spread of ESBL-encoding genes, but not entirely impenetrable.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01459-23","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T08:18:27Z","timestamp":1709626707000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Inter-phylum circulation of a beta-lactamase-encoding gene: a rare but observable event"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7741-9764","authenticated-orcid":true,"given":"R\u00e9mi","family":"Gschwind","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM, Universit\u00e9 Sorbonne Paris Nord, IAME, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Petitjean","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM, Universit\u00e9 Sorbonne Paris Nord, IAME, Paris, France"},{"name":"AP-HP, H\u00f4pital Bichat, Laboratoire de Bact\u00e9riologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudine","family":"Fournier","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland"},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland"},{"name":"INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Lao","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM, Universit\u00e9 Sorbonne Paris Nord, IAME, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Clermont","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM, Universit\u00e9 Sorbonne Paris Nord, IAME, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1343-1622","authenticated-orcid":true,"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland"},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland"},{"name":"INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland"},{"name":"University of Lausanne, University Hospital Center, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Mellmann","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, University Hospital M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7860-1717","authenticated-orcid":true,"given":"Erick","family":"Denamur","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM, Universit\u00e9 Sorbonne Paris Nord, IAME, Paris, France"},{"name":"AP-HP, H\u00f4pital Bichat, Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5160-5286","authenticated-orcid":true,"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 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