{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:26:46Z","timestamp":1761128806524},"reference-count":22,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have evaluated the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of posaconazole (PSC) against 50 clinical strains of\n            <jats:italic>Rhizopus oryzae<\/jats:italic>\n            using a broth microdilution method, the Neo-Sensitabs tablet diffusion method, and minimal fungicidal concentration (MFC) determination. In general, PSC showed low MICs against this fungus, and the MICs correlated with the inhibition zone diameters. Most of the MFCs, however, were from 1 to 4 dilutions higher than their corresponding MICs. We then investigated the efficacies of several different doses of PSC in a murine model. All treatments began 24 h after challenge and lasted for 7 days. The drug was administered twice a day to mice infected with three strains that showed intermediate PSC susceptibility (MIC = 2 \u03bcg\/ml) and three PSC-susceptible strains (MIC = 0.25 \u03bcg\/ml). A dose of 10 mg\/kg of body weight was ineffective, while doses of 20 and 30 mg\/kg prolonged the survival of the mice. The 50 strains tested were segregated into two groups on the basis of the\n            <jats:italic>in vitro<\/jats:italic>\n            data. For the group with the most strains (85%), the strains had low PSC MICs, mice infected with the strains showed higher rates of survival (30 to 40%), and PSC was able to reduce the fungal load in the kidney and less regularly in the brain. For the second group (15% of the strains), the strains had intermediate PSC MICs, mice infected with the strains had lower survival rates (10 to 20%), and PSC treatment resulted in variable and no reductions in the fungal loads in the kidneys and brains, respectively.\n          <\/jats:p>","DOI":"10.1128\/aac.01463-09","type":"journal-article","created":{"date-parts":[[2010,2,10]],"date-time":"2010-02-10T04:54:38Z","timestamp":1265777678000},"page":"1665-1669","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Correlation between\n            <i>In<\/i>\n            <i>Vitro<\/i>\n            Activity of Posaconazole and\n            <i>In<\/i>\n            <i>Vivo<\/i>\n            Efficacy against\n            <i>Rhizopus oryzae<\/i>\n            Infection in Mice"],"prefix":"10.1128","volume":"54","author":[{"given":"M. Mar","family":"Rodri\u0301guez","sequence":"first","affiliation":[{"name":"Unitat de Microbiologia, Facultat de Medicina i Cie\u0300ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Javier","family":"Pastor","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Facultat de Medicina i Cie\u0300ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deanna A.","family":"Sutton","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Calvo","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Facultat de Medicina i Cie\u0300ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette W.","family":"Fothergill","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Salas","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Facultat de Medicina i Cie\u0300ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Rinaldi","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josep","family":"Guarro","sequence":"additional","affiliation":[{"name":"Unitat de Microbiologia, Facultat de Medicina i Cie\u0300ncies de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00452-07"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00036-09"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00969-06"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.11.3647-3650.2003"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00134-07"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01883-07"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3776-3781.2002"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.1.126-133.2006"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01124-08"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1592\/phco.27.12.1627"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00454-06"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00662-08"},{"key":"e_1_3_1_14_2","unstructured":"Reference method for broth dilution antifungal susceptibility testing of filamentous fungi: approved standard 2008"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/514193"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.07.011"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1032-1037.2002"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.643-658.2001"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01026-09"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00628-08"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm429"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/599105"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/500212"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01463-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01463-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:26:28Z","timestamp":1645475188000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01463-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1128\/AAC.01463-09"],"URL":"https:\/\/doi.org\/10.1128\/aac.01463-09","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5]]},"assertion":[{"value":"2009-10-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}