{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:22:03Z","timestamp":1791836523067,"version":"4.3.4"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Assembly Biosciences"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,10,20]]},"abstract":"<jats:p>\n            ABI-H0731, a first-generation hepatitis B virus (HBV) core protein inhibitor, has demonstrated effective antiviral activity in chronic hepatitis B (CHB) patients in a phase 1b clinical trial and is currently being further evaluated in phase 2 clinical trials. Here, we report the preclinical profile of ABI-H0731. In\n            <jats:italic>in vitro<\/jats:italic>\n            cell culture systems (HepG2-derived cell lines HepAD38 and HepG2-NTCP and primary human hepatocytes [PHHs]), ABI-H0731 exhibited selective inhibition of HBV DNA replication (50% effective concentration [EC\n            <jats:sub>50<\/jats:sub>\n            ] from 173\u2009nM to 307\u2009nM).\n          <\/jats:p>","DOI":"10.1128\/aac.01463-20","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T14:26:52Z","timestamp":1598538412000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Preclinical Profile and Characterization of the Hepatitis B Virus Core Protein Inhibitor ABI-H0731"],"prefix":"10.1128","volume":"64","author":[{"given":"Qi","family":"Huang","sequence":"first","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawei","family":"Cai","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Yan","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichun","family":"Li","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Zong","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lida","family":"Guo","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Mercier","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel","family":"Tang","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk","family":"Henne","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5381-3716","authenticated-orcid":false,"given":"Richard","family":"Colonno","sequence":"additional","affiliation":[{"name":"Assembly Biosciences, South San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a021501"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.01.018"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i1.48"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.05.022"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-33"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00082-18"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01734-18"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077215"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00835-18"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00560-17"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep42374"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.60"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.8.1715"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01091-13"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02439-19"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2007.06.014"},{"key":"e_1_3_3_18_2","unstructured":"Chen H Raney AK Allan MJ Song J Lang SA. February 2007. Thiazolidinones oxazolidinones and pyrrolidinones for HBV. 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Continued therapy with ABI-H0731 + NrtI results in sequential reduction\/loss of HBV DNA HBV RNA HBeAg HBcrAg and HBsAg in HBeAg-positive patients abstr LP1. Abstr Am Assoc Study Liver Dis Annu Meet 2019."},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0618-8278(19)30230-0"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-017-0769-2"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006658"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i18.5427"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-052915-015720"},{"key":"e_1_3_3_36_2","first-page":"S271","article-title":"Adherence: a necessity for successful HIV combination therapy","volume":"13","author":"Chesney MA","year":"1999","unstructured":"Chesney MA, Ickovics J, Hecht FM, Sikipa G, Rabkin J. 1999. Adherence: a necessity for successful HIV combination therapy. 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