{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:12:27Z","timestamp":1791295947851,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006251","name":"Universit\u00e9 de Bordeaux","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006251","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A strain of the opportunistic pathogenic yeast\n            <jats:named-content content-type=\"genus-species\">Candida lusitaniae<\/jats:named-content>\n            was genetically modified for use as a cellular model for assessing by allele replacement the impact of lanosterol C14\u03b1-demethylase\n            <jats:italic>ERG11<\/jats:italic>\n            mutations on azole resistance.\n            <jats:named-content content-type=\"genus-species\">Candida lusitaniae<\/jats:named-content>\n            was chosen because it is susceptible to azole antifungals, it belongs to the CTG clade of yeast, which includes most of the\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species pathogenic for humans, and it is haploid and easily amenable to genetic transformation and molecular modeling. In this work, allelic replacement is targeted at the\n            <jats:italic>ERG11<\/jats:italic>\n            locus by the reconstitution of a functional auxotrophic marker in the 3\u2032 intergenic region of\n            <jats:italic>ERG11<\/jats:italic>\n            . Homologous and heterologous\n            <jats:italic>ERG11<\/jats:italic>\n            alleles are expressed from the resident\n            <jats:italic>ERG11<\/jats:italic>\n            promoter of\n            <jats:named-content content-type=\"genus-species\">C. lusitaniae<\/jats:named-content>\n            , allowing accurate comparison of the phenotypic change in azole susceptibility. As a proof of concept, we successfully expressed in\n            <jats:named-content content-type=\"genus-species\">C. lusitaniae<\/jats:named-content>\n            different\n            <jats:italic>ERG11<\/jats:italic>\n            alleles, either bearing or not bearing mutations retrieved from a clinical context, from two phylogenetically distant yeasts,\n            <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Kluyveromyces marxianus<\/jats:named-content>\n            .\n            <jats:named-content content-type=\"genus-species\">Candida lusitaniae<\/jats:named-content>\n            constitutes a high-fidelity expression system, giving specific Erg11p-dependent fluconazole MICs very close to those observed with the\n            <jats:italic>ERG11<\/jats:italic>\n            donor strain. This work led us to characterize the phenotypic effect of two kinds of mutation: mutation conferring decreased fluconazole susceptibility in a species-specific manner and mutation conferring fluconazole resistance in several yeast species. In particular, a missense mutation affecting amino acid K143 of Erg11p in\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species, and the equivalent position K151 in\n            <jats:named-content content-type=\"genus-species\">K. marxianus<\/jats:named-content>\n            , plays a critical role in fluconazole resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.01483-17","type":"journal-article","created":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T00:55:24Z","timestamp":1508201724000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["A CTG Clade Candida Yeast Genetically Engineered for the Genotype-Phenotype Characterization of Azole Antifungal Resistance in Human-Pathogenic Yeasts"],"prefix":"10.1128","volume":"62","author":[{"given":"Isabelle","family":"Accoceberry","sequence":"first","affiliation":[{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, Bordeaux, France"},{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, CHU Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Rougeron","sequence":"additional","affiliation":[{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, Bordeaux, France"},{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, CHU Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Biteau","sequence":"additional","affiliation":[{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Chevrel","sequence":"additional","affiliation":[{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Fitton-Ouhabi","sequence":"additional","affiliation":[{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"No\u00ebl","sequence":"additional","affiliation":[{"name":"University Bordeaux, CNRS, Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, UMR 5234, Bordeaux, 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