{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:33:49Z","timestamp":1792096429148,"version":"4.4.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Beijing Municipal Government"},{"DOI":"10.13039\/501100004147","name":"Tsinghua University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004147","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,12,16]]},"abstract":"<jats:p>\n                    Nucleotide analogs targeting viral RNA polymerase have been proved to be an effective strategy for antiviral treatment and are promising antiviral drugs to combat the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. In this study, we developed a robust\n                    <jats:italic>in vitro<\/jats:italic>\n                    nonradioactive primer extension assay to quantitatively evaluate the efficiency of incorporation of nucleotide analogs by SARS-CoV-2 RNA-dependent RNA polymerase (RdRp). Our results show that many nucleotide analogs can be incorporated into RNA by SARS-CoV-2 RdRp and that the incorporation of some of them leads to chain termination.\n                  <\/jats:p>","DOI":"10.1128\/aac.01508-20","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T10:10:07Z","timestamp":1603966207000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Development of a Simple\n                    <i>In Vitro<\/i>\n                    Assay To Identify and Evaluate Nucleotide Analogs against SARS-CoV-2 RNA-Dependent RNA Polymerase"],"prefix":"10.1128","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2113-5636","authenticated-orcid":false,"given":"Gaofei","family":"Lu","sequence":"first","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weinan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialei","family":"Sun","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Hua","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Xu","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-jie","family":"Chu","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Ding","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"},{"name":"School of Pharmaceutical Sciences, Tsinghua University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Xiong","sequence":"additional","affiliation":[{"name":"Global Health Drug Discovery Institute, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30251-8"},{"key":"e_1_3_3_3_2","volume-title":"Coronavirus disease 2019 (COVID-19): situation report, 88","author":"World Health Organization","year":"2020","unstructured":"World Health Organization. 2020. 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