{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:08:38Z","timestamp":1792652918483,"version":"4.5.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            With the occurrence of extended-spectrum \u03b2-lactamases (ESBLs) in\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            being increasingly reported worldwide, there is a need for a reliable test to detect ESBLs in clinical isolates of\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            . In our study, a total of 75 clinical isolates of\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            were studied. Nitrocefin tests were performed to detect the \u03b2-lactamase enzyme; isoelectric focusing electrophoresis, PCR, and PCR product sequencing were designed to further characterize the contained ESBLs. Various ESBL-screening methods were designed to compare the reliabilities of detecting ESBLs in clinical isolates of\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            whose \u03b2-lactamases were well characterized. Thirty-four of 36 multidrug-resistant\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            clinical isolates were positive for ESBLs.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VEB-3<\/jats:sub>\n            was the most prevalent ESBL gene in\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            in our study. Among the total of 34 isolates that were considered ESBL producers, 20 strains were positive using conventional combined disk tests and 10 strains were positive using a conventional double-disk synergy test (DDST) with amoxicillin-clavulanate, expanded-spectrum cephalosporins, aztreonam, and cefepime. Modifications of the combined disk test and DDST, which consisted of shorter distances between disks (20 mm instead of 30 mm) and the use of three different plates that contained cloxacillin (200 \u03bcg\/ml) alone, Phe-Arg \u03b2-naphthylamide dihydrochloride (MC-207,110; 20 \u03bcg\/ml) alone, and both cloxacillin (200 \u03bcg\/ml) and MC-207,110 (20 \u03bcg\/ml) increased the sensitivity of the tests to 78.8%, 91.18%, 85.29%, and 97.06%.\n          <\/jats:p>","DOI":"10.1128\/aac.01511-05","type":"journal-article","created":{"date-parts":[[2006,8,28]],"date-time":"2006-08-28T18:10:56Z","timestamp":1156788656000},"page":"2990-2995","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Detection of Extended-Spectrum \u03b2-Lactamases in Clinical Isolates of\n            <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"50","author":[{"given":"Xiaofei","family":"Jiang","sequence":"first","affiliation":[{"name":"Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danqiu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyi","family":"Ruan","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.9.3284-3290.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(99)80075-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.11.4228-4232.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.8.1880-1884.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1016-1022.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(00)00152-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00755.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.4.908-911.1996"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.826-831.2005"},{"key":"e_1_3_2_11_2","first-page":"1018","volume":"28","year":"2005","unstructured":"Jiang, X. F., L. Z. Han, F. Y. Yuan, Y. F. Jiang, Y. Liu, Y. Ni, Y. Lu, and X. H. Hong. 2005. Double inhibitor parallel inhibit disk test to effectively analyze ESBLs in Enterobacteriaceae isolates. Chin. J. Lab. Med.28:1018-1021.","journal-title":"Chin. J. Lab. Med."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki160"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_2_14_2","first-page":"281","volume":"35","year":"2001","unstructured":"Livermore, D. M., and D. F. J. Brown. 2001. Detection of \u03b2-lactamase mediated resistance. J. Antimicrob. Chemother.35:281-294.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.105-116.2001"},{"key":"e_1_3_2_16_2","first-page":"1865","volume":"39","year":"2004","unstructured":"Pagani, L., E. Mantengoli, R. Migliavacca, E. Nucleo, S. Pollini, M. Spalla, R. Daturi, E. Romero, and G. M. Rossolini. 2004. Multifocal detection of multidrug-resistant Pseudomonas aeruginosa producing the PER-1 extended-spectrum \u03b2-lactamase in northern Italy. J. Clin. Microbiol.39:1865-1870.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2277-2279.2004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3542-3547.2003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.6.951"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1998.tb00249.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.542-546.2000"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.4059-4062.2004"},{"key":"e_1_3_2_23_2","first-page":"45","volume":"53","year":"2001","unstructured":"Wolska, M. K., K. Bukowski, and A. Jakubczak. 2001. Occurrence of beta-lactamase type ESBL and IBL in Pseudomonas aeruginosa rods. Med. Dosw Mikrobiol.53:45-51.","journal-title":"Med. Dosw Mikrobiol."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01511-05","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01511-05","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:14:21Z","timestamp":1645470861000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01511-05"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":22,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1128\/AAC.01511-05"],"URL":"https:\/\/doi.org\/10.1128\/aac.01511-05","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]},"assertion":[{"value":"2005-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-06-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}