{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T18:15:56Z","timestamp":1783707356475,"version":"3.55.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/22452-1"],"award-info":[{"award-number":["2017\/22452-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/19316-3"],"award-info":[{"award-number":["2015\/19316-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2021,3,18]]},"abstract":"<jats:p>\n            Atovaquone (AV) acts on the malaria parasite by competing with ubiquinol (UQH\n            <jats:sub>2<\/jats:sub>\n            ) for its union to the mitochondrial bc\n            <jats:sub>1<\/jats:sub>\n            complex, preventing the ubiquinone-8 and ubiquinone-9 (UQ-8 and UQ-9) redox recycling, which is a necessary step in pyrimidine biosynthesis. This study focused on UQ biosynthesis in\n            <jats:named-content content-type=\"genus-species\">Plasmodium falciparum<\/jats:named-content>\n            and adopted proof-of-concept research to better elucidate the mechanism of action of AV and improve its efficacy.\n          <\/jats:p>","DOI":"10.1128\/aac.01516-20","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T13:22:14Z","timestamp":1611753734000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Exploring Ubiquinone Biosynthesis Inhibition as a Strategy for Improving Atovaquone Efficacy in Malaria"],"prefix":"10.1128","volume":"65","author":[{"given":"I. B.","family":"Verdaguer","sequence":"first","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2856-5471","authenticated-orcid":true,"given":"M.","family":"Crispim","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. A.","family":"Zafra","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A. C.","family":"Sussmann","sequence":"additional","affiliation":[{"name":"Center for Environmental Sciences, Institute of Humanities, Arts and Sciences, Federal University of Southern Bahia, Itabuna, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. L.","family":"Buritic\u00e1","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. R.","family":"Melo","sequence":"additional","affiliation":[{"name":"Department de Bioscience, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Department de Bioscience, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. G.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. A.","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4782-2162","authenticated-orcid":true,"given":"A. M.","family":"Katzin","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 2019. World Malaria Report. Geneva. https:\/\/www.who.int\/publications\/i\/item\/9789241565721."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-7-S1-S11"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-7646(98)80208-2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073424"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04257.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(79)90094-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/156652410791065390"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00027.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/106002809302701215"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.290973"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05572"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.05049-11"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1054\/drup.2000.0157"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1049"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.6.1334"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/1681080a0"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.281.4.361"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24203721"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762011000900018"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2005.04.002"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(57)90023-8"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90051-K"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm00293a002"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11021.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.10.055"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.372"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-015-2043-0"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030606"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb800145r"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2007.09.006"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-011-9347-6"},{"key":"e_1_3_3_33_2","first-page":"27","volume-title":"Radioisotopes: Applications in Bio-Medical Science","author":"Kimura EA","year":"2011","unstructured":"Kimura EA, Wunderlich G, Jord\u00e3o FM, Tonhosolo R, Gabriel HB, Sussmann RAC, Saito AY, Katzin AM. 2011. Use of radioactive precursors for biochemical characterization the biosynthesis of isoprenoids in intraerythrocytic stages of Plasmodium falciparum., p 27\u201346. In Singh N, Radioisotopes: Applications in Bio-Medical Science. IntechOpen."},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408360200"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1060107"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(72)90255-X"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-50319-5"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.11.005"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2018.07.003"},{"key":"e_1_3_3_40_2","first-page":"107","article-title":"Continuous culture of Plasmodium falciparum asexual stages in \u201cnormal\u201d air atmosphere","volume":"36","author":"Mirovsky P","year":"1989","unstructured":"Mirovsky P. 1989. Continuous culture of Plasmodium falciparum asexual stages in \u201cnormal\u201d air atmosphere. Folia Parasitol (Praha) 36:107\u2013112.","journal-title":"Folia Parasitol (Praha)"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(02)00065-6"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(94)90079-5"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.44.1.14-18.2000"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1996.tb01385.x"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(90)90114-x"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2010.06.007"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0168-9452(00)00200-4"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)81425-l"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.46.9.2889-2894.2002"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00073-12"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04161-14"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.198"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.2307\/3280287"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-6851(97)00237-5"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-7-45"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.48.5.1803-1806.2004"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1997.4194"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0009-3084(00)00227-9"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcq044"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01516-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01516-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:38:09Z","timestamp":1645486689000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01516-20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,18]]},"references-count":58,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,3,18]]}},"alternative-id":["10.1128\/AAC.01516-20"],"URL":"https:\/\/doi.org\/10.1128\/aac.01516-20","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,18]]},"assertion":[{"value":"2020-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01516-20"}}