{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:42:02Z","timestamp":1792114922120,"version":"4.4.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The in vitro susceptibilities of\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            isolates from consecutive human immunodeficiency virus-positive and -negative patients to the antifungal agents fluconazole, amphotericin B, and flucytosine were determined by different techniques, including the CLSI method, Etest, and broth microdilution in yeast nitrogen base (YNB) medium, during a multicenter prospective study in France. The relationship between the in vitro data and the clinical outcome 2 weeks after the initiation of antifungal therapy was assessed. In addition, the correlation between the strain serotype and the in vitro activities of the antifungals was determined, and the susceptibility results obtained with the different techniques were also compared. Thirty-seven patients received a combination of amphotericin B with flucytosine as first-line therapy, 22 were treated with amphotericin B alone, and 15 received fluconazole alone. Whatever the antifungal tested, there was no trend toward higher MICs for strains isolated from patients who failed to respond to a given therapy compared to those from patients who did not with either the CLSI method, Etest, or broth microdilution in YNB medium. The MICs obtained by the CLSI or Etest method were significantly lower for serotype D strains than for serotype A strains for both fluconazole and amphotericin B, while flucytosine MICs were not different according to serotype. These findings suggest that the in vitro antifungal susceptibility of\n            <jats:italic>C. neoformans<\/jats:italic>\n            , as determined with the techniques used, is not able to predict the early clinical outcome in patients with cryptococcosis.\n          <\/jats:p>","DOI":"10.1128\/aac.01520-05","type":"journal-article","created":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T18:26:28Z","timestamp":1151346388000},"page":"2464-2470","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":93,"title":["Results Obtained with Various Antifungal Susceptibility Testing Methods Do Not Predict Early Clinical Outcome in Patients with Cryptococcosis"],"prefix":"10.1128","volume":"50","author":[{"given":"E.","family":"Dannaoui","sequence":"first","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, 75724 Paris Cedex 15, France"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Europe\u0301en Georges Pompidou, Unite\u0301 de Parasitologie-Mycologie, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Abdul","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Arpin","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Mycologie Me\u0301dicale et Pathologie Exotique, Universite\u0301 Claude Bernard Lyon I, 69373 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Michel-Nguyen","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, CHU Timone, and Ho\u0302pital St. Joseph, 13000 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Piens","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Mycologie Me\u0301dicale et Pathologie Exotique, Universite\u0301 Claude Bernard Lyon I, 69373 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Favel","sequence":"additional","affiliation":[{"name":"Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire, Faculte\u0301 de Pharmacie, 13385 Marseille Cedex 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Lortholary","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, 75724 Paris Cedex 15, France"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Necker-Enfants-Malades, Service des Maladies Infectieuses et Tropicales, 75743 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Dromer","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the French Cryptococcosis Study Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1544-1548.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2348-2350.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(99)00137-6"},{"key":"e_1_3_2_5_2","unstructured":"Clinical and Laboratory Standards Institute. 2002. 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