{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:25:09Z","timestamp":1790601909967,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"USAID | President's Malaria Initiative"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,4,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Monitoring antimalarial efficacy is important to detect the emergence of parasite drug resistance. Angola conducts\n                      <jats:italic>in vivo<\/jats:italic>\n                      therapeutic efficacy studies (TESs) every 2 years in its fixed sentinel sites in Benguela, Lunda Sul, and Zaire provinces. Children with uncomplicated\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      malaria were treated with artemether-lumefantrine (AL), artesunate-amodiaquine (ASAQ), dihydroartemisinin-piperaquine (DP), or artesunate-pyronaridine (ASPY) and followed for 28 (AL and ASAQ) or 42 days (DP and ASPY) to assess clinical and parasitological response to treatment. Two drugs were sequentially assessed in each site in February\u2013July 2021. The primary indicator was the Kaplan-Meier estimate of the PCR-corrected efficacy at the end of the follow-up period. A total of 622 patients were enrolled in the study and 590 (95%) participants reached a study endpoint. By day 3, \u226598% of participants were slide-negative in all study sites and arms. After PCR correction, day 28 AL efficacy was 88.0% (95% CI: 82%\u201395%) in Zaire and 94.7% (95% CI: 90%\u201399%) in Lunda Sul. For ASAQ, day 28 efficacy was 92.0% (95% CI: 87%\u201398%) in Zaire and 100% in Lunda Sul. Corrected day 42 efficacy was 99.6% (95% CI: 99%\u2013100%) for ASPY and 98.3% (95% CI: 96%\u2013100%) for DP in Benguela. High day 3 clearance rates suggest no clinical evidence of artemisinin resistance. This was the fourth of five rounds of TES in Angola showing a corrected AL efficacy &lt;90% in a site. For Zaire, AL has had an efficacy &lt;90% in 2013, 2015, and 2021. ASAQ, DP, and ASPY are appropriate choices as artemisinin-based combination therapies in Angola.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01525-23","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T09:00:49Z","timestamp":1709197249000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Therapeutic response to four artemisinin-based combination therapies in Angola, 2021"],"prefix":"10.1128","volume":"68","author":[{"given":"Pedro Rafael","family":"Dimbu","sequence":"first","affiliation":[{"name":"National Malaria Control Program, Ministry of Health, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Labuda","sequence":"additional","affiliation":[{"name":"United States President\u2019s Malaria Initiative, United States Centers for Disease Control and Prevention, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Miguel","family":"Ferreira","sequence":"additional","affiliation":[{"name":"PSI Angola, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felismina","family":"Caquece","sequence":"additional","affiliation":[{"name":"Field Epidemiology Training Program, Ministry of Health, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kialanda","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Field Epidemiology Training Program, Ministry of Health, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garcia","family":"Pembele","sequence":"additional","affiliation":[{"name":"National Institute of Health Research, Ministry of Health, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilunvuidi","family":"Pode","sequence":"additional","affiliation":[{"name":"Field Epidemiology Training Program, Ministry of Health, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Florinda","family":"Jo\u00e3o","sequence":"additional","affiliation":[{"name":"National Malaria Control Program, Ministry of Health, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venceslau Mambi","family":"Pelenda","sequence":"additional","affiliation":[{"name":"PSI Angola, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Nieto Andrade","sequence":"additional","affiliation":[{"name":"PSI Angola, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Breanna","family":"Horton","sequence":"additional","affiliation":[{"name":"United States Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Culzean","family":"Kennedy","sequence":"additional","affiliation":[{"name":"United States Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samaly S.","family":"Svigel","sequence":"additional","affiliation":[{"name":"United States Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Zhou","sequence":"additional","affiliation":[{"name":"United States Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana F. M.","family":"Morais","sequence":"additional","affiliation":[{"name":"PSI Angola, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana do","family":"Ros\u00e1rio","sequence":"additional","affiliation":[{"name":"United States President\u2019s Malaria Initiative, USAID, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomeno","family":"Fortes","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Tropical Medicine, Nova University of Lisbon, Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Franco","family":"Martins","sequence":"additional","affiliation":[{"name":"National Malaria Control Program, Ministry of Health, Luanda, Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7322-4625","authenticated-orcid":true,"given":"Mateusz M.","family":"Plucinski","sequence":"additional","affiliation":[{"name":"United States President\u2019s Malaria Initiative, Malaria Branch, United States Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30261-0"},{"key":"e_1_3_5_4_2","volume-title":"Compendium of WHO malaria guidance: prevention, diagnosis, treatment, surveillance and elimination","author":"World Health Organization","year":"2019","unstructured":"World Health Organization. 2019. Compendium of WHO malaria guidance: prevention, diagnosis, treatment, surveillance and elimination. World Health Organization, Geneva."},{"key":"e_1_3_5_5_2","volume-title":"The use of artesunate-pyronaridine for the treatment of uncomplicated malaria","author":"World Health Organization","year":"2019","unstructured":"World Health Organization. 2019. The use of artesunate-pyronaridine for the treatment of uncomplicated malaria. World Health Organization."},{"key":"e_1_3_5_6_2","unstructured":"World Health Organization. 2022. Strategy to respond to antimalarial drug resistance in Africa. Available from: https:\/\/www.who.int\/publications-detail-redirect\/9789240060265. Retrieved 14 Dec 2023."},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2101746"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(21)00142-0"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-021-03585-6"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.21-0214"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04181-14"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-1712-4"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01949-20"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00207-22"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2290-9"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.20-1481"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-022-04182-x"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.22-0224"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056677"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182099004552"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01228-06"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00072-15"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-11-270"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-8-200"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01242-12"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.2003.02004.x"},{"key":"e_1_3_5_28_2","unstructured":"World Health Organization. Informal consultation on methodology to distinguish reinfection from recrudescence in high malaria transmission areas. Available from: https:\/\/www.who.int\/publications-detail-redirect\/9789240038363. Retrieved 28 Jan 2022."},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30426-8"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00590-19"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01517-19"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.21-0117"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01525-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01525-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T09:07:14Z","timestamp":1712135234000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.01525-23"}},"subtitle":[],"editor":[{"given":"Audrey","family":"Odom John","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,4,3]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4,3]]}},"alternative-id":["10.1128\/aac.01525-23"],"URL":"https:\/\/doi.org\/10.1128\/aac.01525-23","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,3]]},"assertion":[{"value":"2023-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01525-23"}}