{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:42:36Z","timestamp":1791337356896,"version":"4.3.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T00:00:00Z","timestamp":1576800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001828","name":"Erasmus University Rotterdam","doi-asserted-by":"crossref","award":["Eur Fellowship"],"award-info":[{"award-number":["Eur Fellowship"]}],"id":[{"id":"10.13039\/501100001828","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2019,12,20]]},"abstract":"<jats:p>\n            The use of the Sensititre YeastOne YO10 alamarBlue assay for the\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility testing of\n            <jats:named-content content-type=\"genus-species\">Madurella mycetomatis<\/jats:named-content>\n            was evaluated in\n            <jats:italic>M. mycetomatis<\/jats:italic>\n            isolates with and without pyomelanin secretion. Pyomelanin secretion did not influence visual endpoint reading; however, it caused a shift in peak absorbance from 570\u2009nm to 620\u2009nm when read spectrophotometrically. Therefore, when choosing the method for endpoint reading, the presence of pyomelanin should be considered.\n          <\/jats:p>","DOI":"10.1128\/aac.01532-19","type":"journal-article","created":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T13:12:10Z","timestamp":1570713130000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Pyomelanin Secretion in Madurella mycetomatis Interferes with Spectrophotometric Endpoint Reading Using the Sensititre YeastOne alamarBlue Assay but Not with Visual Endpoint Reading"],"prefix":"10.1128","volume":"64","author":[{"given":"B.","family":"Nyuykonge","sequence":"first","affiliation":[{"name":"Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. D.","family":"Croughs","sequence":"additional","affiliation":[{"name":"Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. H.","family":"Fahal","sequence":"additional","affiliation":[{"name":"Mycetoma Research Center, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Verbon","sequence":"additional","affiliation":[{"name":"Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8652-8783","authenticated-orcid":false,"given":"W. W. J.","family":"van de Sande","sequence":"additional","affiliation":[{"name":"Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/trv003"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002550"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00359-X"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myx061"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003218"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.20187075"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.015"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2012.01.007"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002942"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.4.1364-1368.2005"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01170-12"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4535-4540.2005"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.3767\/003158514X684744"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-12-11-1037-RE"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15085807"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701581458"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl526"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01045-10"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2742-2746.2004"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01546-09"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00440"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152796"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01606.x"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.7b01166"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5849(98)00234-2"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01532-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01532-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:39:54Z","timestamp":1645479594000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01532-19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,20]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12,20]]}},"alternative-id":["10.1128\/AAC.01532-19"],"URL":"https:\/\/doi.org\/10.1128\/aac.01532-19","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,20]]},"assertion":[{"value":"2019-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01532-19"}}