{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:34:10Z","timestamp":1788348850141,"version":"build-2803163510"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"crossref","award":["R01AI139179"],"award-info":[{"award-number":["R01AI139179"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"crossref"}]},{"name":"HHS | National Institutes of Health","award":["R01AI075045"],"award-info":[{"award-number":["R01AI075045"]}]},{"DOI":"10.13039\/501100004167","name":"Medicines for Malaria Venture","doi-asserted-by":"crossref","award":["RD\/18-0066"],"award-info":[{"award-number":["RD\/18-0066"]}],"id":[{"id":"10.13039\/501100004167","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,4,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Malaria remains a leading cause of morbidity and mortality in Burkina Faso, which utilizes artemether\u2013lumefantrine as the principal therapy to treat uncomplicated malaria and seasonal malaria chemoprevention with monthly sulfadoxine\u2013pyrimethamine plus amodiaquine in children during the transmission season. Monitoring the activities of available antimalarial drugs is a high priority. We assessed the\n              <jats:italic>ex vivo<\/jats:italic>\n              susceptibility of\n              <jats:italic>Plasmodium falciparum<\/jats:italic>\n              to 11 drugs in isolates from patients presenting with uncomplicated malaria in Bobo-Dioulasso in 2021 and 2022. IC\n              <jats:sub>50<\/jats:sub>\n              values were derived using a standard 72 h growth inhibition assay. Parasite DNA was sequenced to characterize known drug resistance-mediating polymorphisms. Isolates were generally susceptible, with IC\n              <jats:sub>50<\/jats:sub>\n              values in the low-nM range, to chloroquine (median IC\n              <jats:sub>50<\/jats:sub>\n              10 nM, IQR 7.9\u201324), monodesethylamodiaquine (22, 14\u201346) piperaquine (6.1, 3.6\u20139.2), pyronaridine (3.0, 1.3\u20135.5), quinine (50, 30\u201375), mefloquine (7.1, 3.7\u201310), lumefantrine (7.1, 4.5\u201312), dihydroartemisinin (3.7, 2.2\u20135.5), and atovaquone (0.2, 0.1\u20130.3) and mostly resistant to cycloguanil (850, 543\u20131,290) and pyrimethamine (33,200, 18,400\u201354,200), although a small number of outliers were seen. Considering genetic markers of resistance to aminoquinolines, most samples had wild-type PfCRT K76T (87%) and PfMDR1 N86Y (95%) sequences. For markers of resistance to antifolates, established PfDHFR and PfDHPS mutations were highly prevalent, the PfDHPS A613S mutation was seen in 19% of samples, and key markers of high-level resistance (PfDHFR I164L; PfDHPS K540E) were absent or rare (A581G). Mutations in the PfK13 propeller domain known to mediate artemisinin partial resistance were not detected. Overall, our results suggest excellent susceptibilities to drugs now used to treat malaria and moderate, but stable, resistance to antifolates used to prevent malaria.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01534-23","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T14:01:49Z","timestamp":1709042509000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>Ex vivo<\/i>\n            drug susceptibility and resistance mediating genetic polymorphisms of\n            <i>Plasmodium falciparum<\/i>\n            in Bobo-Dioulasso, Burkina Faso"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1530-1493","authenticated-orcid":false,"given":"A. Fabrice","family":"Som\u00e9","sequence":"first","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9984-8030","authenticated-orcid":true,"given":"Melissa D.","family":"Conrad","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachari","family":"Kabr\u00e9","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aminata","family":"Fofana","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Serge","family":"Yerbanga","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"},{"name":"Institut des Sciences et Techniques, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bazi\u00e9","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Neya","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myreille","family":"Som\u00e9","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tegawinde Josue","family":"Kagambega","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Legac","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shreeya","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Bosco","family":"Ou\u00e9draogo","sequence":"additional","affiliation":[{"name":"Institut des Sciences et Techniques, Bobo-Dioulasso, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7953-7622","authenticated-orcid":true,"given":"Philip J.","family":"Rosenthal","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8437-9382","authenticated-orcid":true,"given":"Roland A.","family":"Cooper","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences and Mathematics, Dominican University of California, San Rafael, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","volume-title":"Annuaire Statistique","author":"Minist\u00e8re de la sant\u00e9 et de l\u2019Hygi\u00e8ne publique","year":"2020","unstructured":"Minist\u00e8re de la sant\u00e9 et de l\u2019Hygi\u00e8ne publique. 2020. 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