{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:41:35Z","timestamp":1793256095326,"version":"4.5.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T00:00:00Z","timestamp":1741132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T00:00:00Z","timestamp":1741132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-21-CE35-0002-01"],"award-info":[{"award-number":["ANR-21-CE35-0002-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Regional fund for development","award":["Synergie GY0012082"],"award-info":[{"award-number":["Synergie GY0012082"]}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-10-LABX25-01"],"award-info":[{"award-number":["ANR-10-LABX25-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-11-EQPX-0022"],"award-info":[{"award-number":["ANR-11-EQPX-0022"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,3,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Artemisinin resistance, which poses a serious threat to malaria control efforts, is monitored in the field by delayed parasite clearance in patients, elevated parasite survival rate in the ring-stage survival assay, and mutations in the\n              <jats:italic>Plasmodium falciparum kelch13<\/jats:italic>\n              gene. However, sporadic cases of artemisinin-resistant malaria do not meet all of these criteria, highlighting that our understanding of artemisinin resistance is still incomplete. Here, we selected for artemisinin resistance\n              <jats:italic>in vitro<\/jats:italic>\n              in nine\n              <jats:italic>P. falciparum<\/jats:italic>\n              field isolates from Africa, Asia, and South America. Artemisinin pressure selected highly resistant parasites in all lineages, but only one acquired a validated\n              <jats:italic>pfkelch13<\/jats:italic>\n              mutation. Phenotypic tests evidenced an unconventional response of selected parasites that can be artemisinin-resistant only at an advanced ring age, making them undetectable by the standard \u201c0\u20133 h\u201d test used to monitor resistance. These results highlight the need for broader approaches to artemisinin resistance monitoring.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01541-24","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T14:00:27Z","timestamp":1738677627000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Artemisinin pressure in field isolates can select highly resistant\n            <i>Plasmodium falciparum<\/i>\n            parasites with unconventional phenotype and no K13 mutation"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0359-3959","authenticated-orcid":true,"given":"Lucie","family":"Paloque","sequence":"first","affiliation":[{"name":"LCC-CNRS, Laboratoire de Chimie de Coordination, Universit\u00e9 de Toulouse, CNRS","place":["Toulouse, France"]},{"name":"MAAP, New antimalarial molecules and pharmacological approaches, Inserm ERL 1289","place":["Toulouse, France"]},{"name":"Institut de Pharmacologie et de Biologie Structurale (IPBS), Universit\u00e9 de Toulouse, CNRS, Universit\u00e9 Toulouse III \u2013 Paul Sabatier","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana","family":"Mathieu","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Centre National de R\u00e9f\u00e9rence du Paludisme, P\u00f4le Zones End\u00e9miques, WHO Collaborating Center for Surveillance of Antimalarial Drug Resistance, Institut Pasteur de la Guyane","place":["Cayenne, French Guiana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Laurent","sequence":"additional","affiliation":[{"name":"LCC-CNRS, Laboratoire de Chimie de Coordination, Universit\u00e9 de Toulouse, CNRS","place":["Toulouse, France"]},{"name":"MAAP, New antimalarial molecules and pharmacological approaches, Inserm ERL 1289","place":["Toulouse, France"]},{"name":"Institut de Pharmacologie et de Biologie Structurale (IPBS), Universit\u00e9 de Toulouse, CNRS, Universit\u00e9 Toulouse III \u2013 Paul Sabatier","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Copp\u00e9e","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universit\u00e9 de Rouen Normandie","place":["Mont-Saint-Aignan, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Blandin","sequence":"additional","affiliation":[{"name":"CNRS, Inserm, UPR9022\/U1257, Mosquito Immune Responses (MIR), Universit\u00e9 de Strasbourg","place":["Strasbourg, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Campagne","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Bioinformatics and Biostatistics Hub, Universit\u00e9 Paris Cit\u00e9","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Augereau","sequence":"additional","affiliation":[{"name":"LCC-CNRS, Laboratoire de Chimie de Coordination, Universit\u00e9 de Toulouse, CNRS","place":["Toulouse, France"]},{"name":"MAAP, New antimalarial molecules and pharmacological approaches, Inserm ERL 1289","place":["Toulouse, France"]},{"name":"Institut de Pharmacologie et de Biologie Structurale (IPBS), Universit\u00e9 de Toulouse, CNRS, Universit\u00e9 Toulouse III \u2013 Paul Sabatier","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise","family":"Musset","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Centre National de R\u00e9f\u00e9rence du Paludisme, P\u00f4le Zones End\u00e9miques, WHO Collaborating Center for Surveillance of Antimalarial Drug Resistance, Institut Pasteur de la Guyane","place":["Cayenne, French Guiana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9468-4823","authenticated-orcid":true,"given":"Fran\u00e7oise","family":"Benoit-Vical","sequence":"additional","affiliation":[{"name":"LCC-CNRS, Laboratoire de Chimie de Coordination, Universit\u00e9 de Toulouse, CNRS","place":["Toulouse, France"]},{"name":"MAAP, New antimalarial molecules and pharmacological approaches, Inserm ERL 1289","place":["Toulouse, France"]},{"name":"Institut de Pharmacologie et de Biologie Structurale (IPBS), Universit\u00e9 de Toulouse, CNRS, Universit\u00e9 Toulouse III \u2013 Paul Sabatier","place":["Toulouse, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 2023. 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