{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:20:44Z","timestamp":1790486444304,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Beijing Natural Science Foundation","award":["7172157"],"award-info":[{"award-number":["7172157"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2016yfc1200100"],"award-info":[{"award-number":["2016yfc1200100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The use of azole fungicides in agriculture is believed to be one of the main reasons for the emergence of azole resistance in\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            . Though widely used in agriculture, imidazole fungicides have not been linked to resistance in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . This study showed that elevated MIC values of imidazole drugs were observed against\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            isolates with TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H\/S297T\/F495I mutation, but not among isolates with TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H mutation. Short-tandem-repeat (STR) typing analysis of 580\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            isolates from 20 countries suggested that the majority of TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H\/S297T\/F495I strains from China were genetically different from the predominant major clade comprising most of the azole-resistant strains and the strains with the same mutation from the Netherlands and Denmark. Alignments of sterol 14\u03b1-demethylase sequences suggested that F495I in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            was orthologous to F506I in\n            <jats:named-content content-type=\"genus-species\">Penicillium digitatum<\/jats:named-content>\n            and F489L in\n            <jats:named-content content-type=\"genus-species\">Pyrenophora teres<\/jats:named-content>\n            , which have been reported to be associated with imidazole resistance.\n            <jats:italic>In vitro<\/jats:italic>\n            antifungal susceptibility testing of different recombinants with\n            <jats:italic>cyp51A<\/jats:italic>\n            mutations further confirmed the association of the F495I mutation with imidazole resistance. In conclusion, this study suggested that environmental use of imidazole fungicides might confer selection pressure for the emergence of azole resistance in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.01549-17","type":"journal-article","created":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T13:05:29Z","timestamp":1519909529000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Elevated MIC Values of Imidazole Drugs against Aspergillus fumigatus Isolates with TR\n            <sub>34<\/sub>\n            \/L98H\/S297T\/F495I Mutation"],"prefix":"10.1128","volume":"62","author":[{"given":"Yong","family":"Chen","sequence":"first","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongwei","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelin","family":"Han","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuguang","family":"Tian","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingya","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Su","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Dermatology Department, Tongji Hospital of Tongji University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziying","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, General Hospital of Chengdu Military Command, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanwen","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, General Hospital of Ji'nan Military Command, Ji'nan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Chinese PLA 302 Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangbin","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Chinese PLA 202 Hospital, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyao","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Jia","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyi","family":"Lu","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandong","family":"Hu","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuyu","family":"Huang","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Verweij","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Han","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2010.10.003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-1944-3"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ885"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12440"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050219"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit320"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01005-16"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2016.12.013"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031801"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05088-11"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70265-8"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30138-4"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01599-09"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks160"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00100-15"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052871"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00003-15"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00185-11"},{"key":"e_1_3_3_20_2","volume-title":"Risk assessment on the impact of environmental usage of triazoles on the development and spread of resistance to medical triazoles in Aspergillus species","author":"European Centre for Disease Prevention and Control","year":"2013","unstructured":"European Centre for Disease Prevention and Control. 2013. Risk assessment on the impact of environmental usage of triazoles on the development and spread of resistance to medical triazoles in Aspergillus species. ECDC, Stockholm, Sweden."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4607"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2015.11.009"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-014-4112-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01279"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3355-7"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02159-07"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2011.08.002"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.06.001"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3744"},{"key":"e_1_3_3_30_2","unstructured":"Arendrup MC Guinea J Cuenca-Estrella M Meletiadis J Mouton JW Lagrou K Howard SJ; Subcommittee on Antifungal Susceptibility Testing of the ESCMID European Committee for Antimicrobial Susceptibility Testing. 22 December 2015. EUCAST Definitive Document EDEF 9.3. Method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia forming moulds. http:\/\/www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/AFST\/Files\/EUCAST_E_Def_9_3_Mould_testing_definitive.pdf."},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku566"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku259"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02855-14"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku410"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00514-12"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2014.05.009"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12089"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00213-11"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv177"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12324"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.02.011"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4112-4120.2005"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01358-10"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12354"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00428"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000255"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.04.001"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2010.540229"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01159-11"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12494"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2016.01.015"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02423-06"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1086\/648452"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.08.029"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01105-14"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01549-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01549-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:13:59Z","timestamp":1645478039000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01549-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5]]},"references-count":54,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1128\/AAC.01549-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.01549-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5]]},"assertion":[{"value":"2017-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01549-17"}}