{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:30:12Z","timestamp":1788489012408,"version":"build-2803163510"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Amiens Hospital","award":["PHRC ERC"],"award-info":[{"award-number":["PHRC ERC"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An interregional surveillance program was conducted in the northwestern part of France to determine the prevalence of carbapenem-nonsusceptible\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            (CNSE) isolates and their susceptibility to ceftazidime-avibactam and aztreonam-avibactam combinations. Nonduplicate CNSE clinical isolates were prospectively collected from six hospitals between June 2012 and November 2013. MICs of ceftazidime and aztreonam, alone or combined with a fixed concentration of avibactam (4 \u03bcg\/ml), and those of carbapenems (comparator agents) were determined. MICs of ertapenem in combination with phenylalanine arginine-naphthylamide dihydrochloride (PA\u03b2N) were also determined to assess active efflux. Genes encoding carbapenemases, plasmid-mediated AmpC enzymes, extended-spectrum \u03b2-lactamases (ESBLs), and major outer membrane proteins (OMPs) were amplified and sequenced. OMPs were also extracted for SDS-PAGE analysis. Among the 139 CNSE isolates, mainly\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacter<\/jats:named-content>\n            spp. and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            , 123 (88.4%) were ertapenem nonsusceptible, 12 (8.6%) exhibited reduced susceptibility to all carbapenems, and 4\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Proteeae<\/jats:named-content>\n            isolates (2.9%) were resistant to imipenem. Carbapenemase production was detected in only two isolates (producing OXA-48 and IMI-3). In contrast, OMP deficiency, in association with AmpCs and\/or ESBLs (mainly CTX-M-9, SHV-12, and CTX-M-15), was largely identified among CNSE isolates. The ceftazidime-avibactam and aztreonam-avibactam combinations exhibited potent activity against CNSE isolates (MIC\n            <jats:sub>50<\/jats:sub>\n            \/MIC\n            <jats:sub>90<\/jats:sub>\n            , 1\/1 \u03bcg\/ml and 0.5\/0.5 \u03bcg\/ml, respectively) compared to that of ceftazidime and aztreonam alone (MIC\n            <jats:sub>50<\/jats:sub>\n            \/MIC\n            <jats:sub>90<\/jats:sub>\n            , 512\/512 \u03bcg\/ml and 128\/512 \u03bcg\/ml, respectively). This study reveals the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of ceftazidime-avibactam and aztreonam-avibactam combinations against a large collection of porin-deficient enterobacterial isolates that are representative of the CNSE recovered in the northern part of France.\n          <\/jats:p>","DOI":"10.1128\/aac.01559-15","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T12:34:21Z","timestamp":1445344461000},"page":"215-221","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Molecular Characterization of Carbapenem-Nonsusceptible Enterobacterial Isolates Collected during a Prospective Interregional Survey in France and Susceptibility to the Novel Ceftazidime-Avibactam and Aztreonam-Avibactam Combinations"],"prefix":"10.1128","volume":"60","author":[{"given":"Herv\u00e9","family":"Dupont","sequence":"first","affiliation":[{"name":"D\u00e9partement d'Anesth\u00e9sie-R\u00e9animation, CHU Amiens-Picardie, Amiens, France, and INSERM U1088, Universit\u00e9 de Picardie Jules Verne, Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Gaillot","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, P\u00f4le de Biologie Pathologie G\u00e9n\u00e9tique du CHU de Lille, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Goetgheluck","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale, H\u00f4pital d'Abbeville, Abbeville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Plassart","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie M\u00e9dicale, Centre Hospitalier de Beauvais, Beauvais, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Emond","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie M\u00e9dicale, H\u00f4pital de Compi\u00e8gne, Compi\u00e8gne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Lecuru","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, H\u00f4pital Sud, Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, H\u00f4pital Sud, Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Derdouri","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, H\u00f4pital Sud, Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baptiste","family":"Lemaire","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, H\u00f4pital Sud, Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Girard de Courtilles","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, H\u00f4pital Sud, Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Cattoir","sequence":"additional","affiliation":[{"name":"CHU de Caen, Service de Microbiologie, Caen, France, and Universit\u00e9 de Caen Basse Normandie, EA 4655 (\u00c9quipe Antibio-R\u00e9sistance), Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hedi","family":"Mammeri","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Centre Hospitalo-Universitaire d'Amiens, H\u00f4pital Sud, Amiens, France"},{"name":"INSERM, IAME, UMR 1137, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01762-09"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.8.3203-3206.2004"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00072-07"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir202"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","DOI":"10.2807\/ese.16.22.19880-en","article-title":"Emergence of carbapenemase-producing Enterobacteriaceae in France, 2004 to 2011","volume":"16","author":"Vaux S","year":"2011","unstructured":"Vaux S, Carbonne A, Thiolet JM, Jarlier V, Coignard B, RAISIN and Expert Laboratories Groups. 2011. 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