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The isolate produced a 5,362-Da bacteriocin (enterocin) that inhibits the growth of\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Enteritidis,\n            <jats:italic>S. enterica<\/jats:italic>\n            serovar Choleraesuis,\n            <jats:italic>S. enterica<\/jats:italic>\n            serovar Typhimurium,\n            <jats:italic>S. enterica<\/jats:italic>\n            serovar Gallinarum,\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O157:H7,\n            <jats:italic>Yersinia enterocolitica<\/jats:italic>\n            ,\n            <jats:italic>Citrobacter freundii<\/jats:italic>\n            ,\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            ,\n            <jats:italic>Shigella dysenteriae<\/jats:italic>\n            ,\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            ,\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            ,\n            <jats:italic>Morganella morganii<\/jats:italic>\n            ,\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            ,\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            ,\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            ,\n            <jats:italic>Campylobacter jejuni<\/jats:italic>\n            , and 20 other\n            <jats:italic>Campylobacter<\/jats:italic>\n            species isolates. The enterocin, E-760, was isolated and purified by cation-exchange and hydrophobic-interaction chromatographies. The proteinaceous nature of purified enterocin E-760 was demonstrated upon treatment with various proteolytic enzymes. Specifically, the antimicrobial peptide was found to be sensitive to beta-chymotrypsin, proteinase K, and papain, while it was resistant to lysozyme and lipase. The enterocin demonstrated thermostability by retaining activity after 5 min at 100\u00b0C and was stable at pH values between 5.0 and 8.7. However, activity was lost below pH 3.0 and above pH 9.5. Administration of enterocin E-760-treated feed significantly (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05) reduced the colonization of young broiler chicks experimentally challenged and colonized with two strains of\n            <jats:italic>C. jejuni<\/jats:italic>\n            by more than 8 log\n            <jats:sub>10<\/jats:sub>\n            CFU. Enterocin E-760 also significantly (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05) reduced the colonization of naturally acquired\n            <jats:italic>Campylobacter<\/jats:italic>\n            species in market age broiler chickens when administered in treated feed 4 days prior to analysis.\n          <\/jats:p>","DOI":"10.1128\/aac.01569-06","type":"journal-article","created":{"date-parts":[[2007,12,18]],"date-time":"2007-12-18T02:39:01Z","timestamp":1197945541000},"page":"1094-1100","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":185,"title":["Isolation and Purification of Enterocin E-760 with Broad Antimicrobial Activity against Gram-Positive and Gram-Negative Bacteria"],"prefix":"10.1128","volume":"52","author":[{"given":"J. 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