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The most potent compound (compound 22; 1-[(\n            <jats:italic>n<\/jats:italic>\n            -decylsulfonyl)ethyl]-1,1-bisphosphonic acid) is a sulfone-containing compound, which had a 50% effective concentration (EC\n            <jats:sub>50<\/jats:sub>\n            ) of 0.11 \u00b1 0.02 \u03bcM against intracellular tachyzoites. The compound showed low toxicity when tested in tissue culture with a selectivity index of &gt;2,000. Compound 22 also showed high activity\n            <jats:italic>in vivo<\/jats:italic>\n            in a toxoplasmosis mouse model. The compound inhibited the\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            farnesyl diphosphate synthase (\n            <jats:italic>Tg<\/jats:italic>\n            FPPS), but the concentration needed to inhibit 50% of the enzymatic activity (IC\n            <jats:sub>50<\/jats:sub>\n            ) was higher than the concentration that inhibited 50% of growth. We tested compound 22 against two other apicomplexan parasites,\n            <jats:named-content content-type=\"genus-species\">Plasmodium falciparum<\/jats:named-content>\n            (EC\n            <jats:sub>50<\/jats:sub>\n            of 0.6 \u00b1 0.01 \u03bcM), the agent of malaria, and\n            <jats:named-content content-type=\"genus-species\">Cryptosporidium parvum<\/jats:named-content>\n            (EC\n            <jats:sub>50<\/jats:sub>\n            of \u223c65 \u03bcM), the agent of cryptosporidiosis. Our results suggest that compound 22 is an excellent novel compound that could lead to the development of potent agents against apicomplexan parasites.\n          <\/jats:p>","DOI":"10.1128\/aac.01590-16","type":"journal-article","created":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T02:31:07Z","timestamp":1480386667000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["<i>In Vitro<\/i>\n            and\n            <i>In Vivo<\/i>\n            Activities of Sulfur-Containing Linear Bisphosphonates against Apicomplexan Parasites"],"prefix":"10.1128","volume":"61","author":[{"given":"Sergio H.","family":"Szajnman","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica and UMYMFOR (CONICET-FCEyN), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamila","family":"Galaka","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica and UMYMFOR (CONICET-FCEyN), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhu-Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan M.","family":"Howell","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda N.","family":"Chao","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica and UMYMFOR (CONICET-FCEyN), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Striepen","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasant","family":"Muralidharan","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia N. 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