{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:28:34Z","timestamp":1787016514958,"version":"build-2736575974"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n A unique\n Klebsiella<\/jats:named-content>\n species strain, 10982, was cultured from a perianal swab specimen obtained from a patient in the University of Maryland Medical Center intensive care unit.\n Klebsiella<\/jats:named-content>\n sp. 10982 possesses a large IncA\/C multidrug resistance plasmid encoding a novel FOX AmpC \u03b2-lactamase designated FOX-10. A novel variant of the LEN \u03b2-lactamase was also identified. Genome sequencing and bioinformatic analysis demonstrated that this isolate contains genes associated with nitrogen fixation, allantoin metabolism, and citrate fermentation. These three gene regions are typically present in either\n Klebsiella pneumoniae<\/jats:named-content>\n clinical isolates or\n Klebsiella<\/jats:named-content>\n nitrogen-fixing endophytes but usually not in the same organism. Phylogenomic analysis of\n Klebsiella<\/jats:named-content>\n sp. 10982 and sequenced\n Klebsiella<\/jats:named-content>\n genomes demonstrated that\n Klebsiella<\/jats:named-content>\n sp. 10982 is present on a branch that is located intermediate between the genomes of nitrogen-fixing endophytes and\n K. pneumoniae<\/jats:named-content>\n clinical isolates. Metabolic features identified in the genome of\n Klebsiella<\/jats:named-content>\n sp. 10982 distinguish this isolate from other\n Klebsiella<\/jats:named-content>\n clinical isolates. These features include the nitrogen fixation (\n nif<\/jats:italic>\n ) gene cluster, which is typically present in endophytic\n Klebsiella<\/jats:named-content>\n isolates and is absent from\n Klebsiella<\/jats:named-content>\n clinical isolates. Additionally, the\n Klebsiella<\/jats:named-content>\n sp. 10982 genome contains genes associated with allantoin metabolism, which have been detected primarily in\n K. pneumoniae<\/jats:named-content>\n isolates from liver abscesses. Comparative genomic analysis of\n Klebsiella<\/jats:named-content>\n sp. 10982 demonstrated that this organism has acquired genes conferring new metabolic strategies and novel antibiotic resistance alleles, both of which may enhance its ability to colonize the human body.\n <\/jats:p>","DOI":"10.1128\/aac.01605-13","type":"journal-article","created":{"date-parts":[[2014,1,6]],"date-time":"2014-01-06T22:18:30Z","timestamp":1389046710000},"page":"1879-1888","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Characterization of Klebsiella sp. 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