{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:01:19Z","timestamp":1791414079836,"version":"4.3.3"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000038","name":"U.S. Food and Drug Administration","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Plazomicin, a next-generation aminoglycoside, was recently approved by the U.S. Food and Drug Administration for the treatment of multidrug-resistant complicated urinary tract infections. It is highly effective against most aminoglycoside-modifying enzymes (AMEs). Here, we report that\n              <jats:italic toggle=\"yes\">Salmonella enterica<\/jats:italic>\n              strains containing the newly identified gentamicin resistance gene (\n              <jats:italic toggle=\"yes\">grdA<\/jats:italic>\n              ) are highly resistant to plazomicin. Heterologous expression of\n              <jats:italic toggle=\"yes\">grdA<\/jats:italic>\n              in\n              <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n              \u0394\n              <jats:italic toggle=\"yes\">tolC<\/jats:italic>\n              resulted in plazomicin resistance with minimum inhibitory concentration (MIC) &gt; 256 \u00b5g\/mL. These findings reveal that GrdA confers significantly higher resistance to plazomicin than the previously known plazomicin-resistant AMEs AA (2)-Ia and APH (2\u2033)-Iva.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01606-24","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T12:06:37Z","timestamp":1743768397000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["The newly identified\n            <i>grdA<\/i>\n            gene confers high-level plazomicin resistance in\n            <i>Salmonella enterica<\/i>\n            serovars"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7707-5332","authenticated-orcid":false,"given":"Ahmed F.","family":"Hikal","sequence":"first","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration","place":["Jefferson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration","place":["Laurel, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3146-4548","authenticated-orcid":true,"given":"Steven","family":"Foley","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration","place":["Jefferson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9503-5393","authenticated-orcid":true,"given":"Ashraf A.","family":"Khan","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration","place":["Jefferson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2016.02.011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-821776-4.00008-1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00867-20"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.8b00001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4792"},{"key":"e_1_3_2_8_2","series-title":"CLSI Supplement M100","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"CLSI","year":"2023","unstructured":"CLSI. 2023. Performance standards for antimicrobial susceptibility testing. CLSI Supplement M100. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.042572.113"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.15.4303-4310.1995"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2741\/macinga"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1229"},{"key":"e_1_3_2_13_2","volume-title":"Enterococci: From Commensals to Leading Causes of Drug Resistant Infection","author":"Gilmore MS","year":"2014","unstructured":"Gilmore MS, Clewell DB, Ike Y, Shankar N, eds. 2014. Enterococci: From Commensals to Leading Causes of Drug Resistant Infection. Eye and Ear Infirmary, Massachusetts."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01606-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01606-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T13:01:55Z","timestamp":1746622915000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.01606-24"}},"subtitle":[],"editor":[{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,5,7]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5,7]]}},"alternative-id":["10.1128\/aac.01606-24"],"URL":"https:\/\/doi.org\/10.1128\/aac.01606-24","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,7]]},"assertion":[{"value":"2024-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01606-24"}}