{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:47:05Z","timestamp":1791150425424,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The genus<jats:italic>Chryseobacterium<\/jats:italic>and other genera belonging to the family<jats:italic>Flavobacteriaceae<\/jats:italic>include organisms that can behave as human pathogens and are known to cause different kinds of infections. Several species of<jats:italic>Flavobacteriaceae<\/jats:italic>, including<jats:italic>Chryseobacterium indologenes<\/jats:italic>, are naturally resistant to \u03b2-lactam antibiotics (including carbapenems), due to the production of a resident metallo-\u03b2-lactamase. Although<jats:italic>C. indologenes<\/jats:italic>presently constitutes a limited clinical threat, the incidence of infections caused by this organism is increasing in some settings, where isolates that exhibit multidrug resistance phenotypes (including resistance to aminoglycosides and quinolones) have been detected. Here, we report the identification and characterization of a new IND-type variant from a<jats:italic>C. indologenes<\/jats:italic>isolate from Burkina Faso that is resistant to \u03b2-lactams and aminoglycosides. The levels of sequence identity of the new variant to other IND-type metallo-\u03b2-lactamases range between 72 and 90% (for IND-4 and IND-5, respectively). The purified enzyme exhibited N-terminal heterogeneity and a posttranslational modification consisting of the presence of a pyroglutamate residue at the N terminus. IND-6 shows a broad substrate profile, with overall higher turnover rates than IND-5 and higher activities than IND-2 and IND-5 against ceftazidime and cefepime.<\/jats:p>","DOI":"10.1128\/aac.01607-08","type":"journal-article","created":{"date-parts":[[2009,8,4]],"date-time":"2009-08-04T01:41:09Z","timestamp":1249350069000},"page":"4320-4326","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["IND-6, a Highly Divergent IND-Type Metallo-\u03b2-Lactamase from<i>Chryseobacterium indologenes<\/i>Strain 597 Isolated in Burkina Faso"],"prefix":"10.1128","volume":"53","author":[{"given":"Boukare\u0301","family":"Zeba","sequence":"first","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Applique\u0301e (UFR-SVT), University of Ouagadougou, 03 BP 7021 Ouagadougou, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"De Luca","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Dubus","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines et Laboratoire d'Enzymologie, Universite\u0301 de Lie\u0300ge, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Delmarcelle","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines et Laboratoire d'Enzymologie, Universite\u0301 de Lie\u0300ge, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Simpore\u0301","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Applique\u0301e (UFR-SVT), University of Ouagadougou, 03 BP 7021 Ouagadougou, Burkina Faso"},{"name":"Saint Camille Medical Center, 01 BP 364 Ouagadougou, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odile G.","family":"Nacoulma","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie et de Biochimie Applique\u0301e (UFR-SVT), University of Ouagadougou, 03 BP 7021 Ouagadougou, Burkina Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Fre\u0300re","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines et Laboratoire d'Enzymologie, Universite\u0301 de Lie\u0300ge, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Denis","family":"Docquier","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705261"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti770"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/508839"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1878-1886.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.10.3223-3227.2002"},{"key":"e_1_3_2_7_2","first-page":"127","volume":"171","year":"1999","unstructured":"Bellais, S., S. 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