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Both liver and blood phases of\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      infection are essential for disease progression and transmission, making them primary targets for pharmacological intervention. In this study, we established a dual-luciferase reporter assay to simultaneously assess\n                      <jats:italic toggle=\"yes\">Plasmodium berghei<\/jats:italic>\n                      liver-stage development and host cell viability. Screening a library of bioactive small molecules with annotated human targets revealed several inhibitors with potent liver-stage activity and minimal cytotoxicity. Further profiling against\n                      <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                      asexual blood stages identified compounds with dual-stage activity, underscoring their potential utility for development as either prophylactic or therapeutic agents. Knowledge of their human targets will facilitate investigation into host- or parasite-directed antimalarial mechanisms, thereby accelerating both biological insight and rational drug development. Our findings constitute a valuable resource for research on malaria biology and the discovery of next-generation antiplasmodials.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01613-25","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:00:17Z","timestamp":1791205217000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Screening of a chemogenetic library unveils dual-stage antiplasmodials with low cytotoxicity"],"prefix":"10.1128","author":[{"given":"Laura","family":"Torres","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Open Innovation at Global Health Disease Area, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew E.","family":"Fishbaugher","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Global Health Disease Area, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Lam","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Open Innovation at Global Health Disease Area, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander T.","family":"Chao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Global Health Disease Area, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calla","family":"Martyn","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Discovery Sciences, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luying","family":"Pei","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Global Health Disease Area, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debapriya","family":"Sengupta","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Global Health Disease Area, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Dauphinais","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Global Discovery Chemistry, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan E.","family":"Gable","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053gv2m95","id-type":"ROR","asserted-by":"publisher"}],"name":"Global Health Disease Area, Biomedical Research, Novartis","place":["Emeryville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7156-5725","authenticated-orcid":true,"given":"Srinivasa P. 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