{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:28:49Z","timestamp":1787016529975,"version":"build-2736575974"},"reference-count":73,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chagas' disease, a neglected tropical illness for which current therapy is unsatisfactory, is caused by the intracellular parasite\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    . The goal of this work is to investigate the\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    effects of the arylimidamide (AIA) DB766 against\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    . This arylimidamide exhibits strong trypanocidal activity and excellent selectivity for bloodstream trypomastigotes and intracellular amastigotes (Y strain), giving IC\n                    <jats:sub>50<\/jats:sub>\n                    s (drug concentrations that reduce 50% of the number of the treated parasites) of 60 and 25 nM, respectively. DB766 also exerts striking effects upon different parasite stocks, including those naturally resistant to benznidazole, and displays higher activity\n                    <jats:italic>in vitro<\/jats:italic>\n                    than the reference drugs. By fluorescent and transmission electron microscopy analyses, we found that this AIA localizes in DNA-enriched compartments and induces considerable damage to the mitochondria. DB766 effectively reduces the parasite load in the blood and cardiac tissue and presents efficacy similar to that of benznidazole in mouse models of\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    infection employing the Y and Colombian strains, using oral and intraperitoneal doses of up to 100 mg\/kg\/day that were given after the establishment of parasite infection. This AIA ameliorates electrocardiographic alterations, reduces hepatic and heart lesions induced by the infection, and provides 90 to 100% protection against mortality, which is similar to that provided by benznidazole. Our data clearly show the trypanocidal efficacy of DB766, suggesting that this AIA may represent a new lead compound candidate to Chagas' disease treatment.\n                  <\/jats:p>","DOI":"10.1128\/aac.01617-09","type":"journal-article","created":{"date-parts":[[2010,5,11]],"date-time":"2010-05-11T00:10:35Z","timestamp":1273536635000},"page":"2940-2952","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Arylimidamide DB766, a Potential Chemotherapeutic Candidate for Chagas' Disease Treatment"],"prefix":"10.1128","volume":"54","author":[{"given":"Denise da Gama Jae\u0301n","family":"Batista","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Meuser","family":"Batista","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Melo de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patri\u0301cia Borges do","family":"Amaral","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseli","family":"Lannes-Vieira","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia das Interac\u0327o\u0303es"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constanc\u0327a Carvalho","family":"Britto","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio Biologia Molecular e Doenc\u0327as Ende\u0302micas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Junqueira","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Doenc\u0327as Parasita\u0301rias"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marli Maria","family":"Lima","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Eco-epidemiologia da doenc\u0327a de Chagas, Instituto Oswaldo Cruz, Fundac\u0327a\u0303o Oswaldo Cruz, Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro Jose\u0301","family":"Romanha","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Parasitologia Celular e Molecular, Centro de Pesquisas Rene\u0301 Rachou, Fiocruz-MG, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Policarpo Ademar","family":"Sales Junior","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Parasitologia Celular e Molecular, Centro de Pesquisas Rene\u0301 Rachou, Fiocruz-MG, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad E.","family":"Stephens","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Augusta State University, Augusta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Boykin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Georgia State University, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria de Nazare\u0301 Correia","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762007005000098"},{"key":"e_1_3_2_3_2","unstructured":"Historia Natural da Doen\u00e7a de Chagas no Estado do Cear\u00e1. 1987"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)90035-X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3150631"},{"key":"e_1_3_2_6_2","first-page":"1","volume":"21","year":"2009","unstructured":"Batista, D. 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