{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:03:28Z","timestamp":1792134208369,"version":"4.4.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,4,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic>Sporothrix brasiliensis<\/jats:italic>\n                      is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61\n                      <jats:italic>Sporothrix<\/jats:italic>\n                      spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28\/56) of\n                      <jats:italic>S. brasiliensis<\/jats:italic>\n                      isolates showing elevated itraconazole minimum inhibitory concentrations \u226516 \u00b5g\/mL. Phylogenetic analysis found the\n                      <jats:italic>in vitro<\/jats:italic>\n                      resistant isolates were not clonal and were distributed across three different\n                      <jats:italic>S. brasiliensis<\/jats:italic>\n                      clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of\n                      <jats:italic>in vitro<\/jats:italic>\n                      resistance. Two of the 28 resistant isolates (MIC \u226516 mg\/L) had a polymorphism in the cytochrome P450 gene,\n                      <jats:italic>cyp51<\/jats:italic>\n                      , corresponding to the well-known G448S substitution inducing azole resistance in\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      . SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26\n                      <jats:italic>in vitro<\/jats:italic>\n                      resistant isolates.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01620-23","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T09:00:39Z","timestamp":1708592439000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Bimodal distribution of azole susceptibility in\n                    <i>Sporothrix brasiliensis<\/i>\n                    isolates in Brazil"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5577-4498","authenticated-orcid":true,"given":"Amanda","family":"Ribeiro dos Santos","sequence":"first","affiliation":[{"name":"Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA"},{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalitha","family":"Gade","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6243-7716","authenticated-orcid":true,"given":"Elizabeth","family":"Misas","sequence":"additional","affiliation":[{"name":"Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA"},{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1086-8041","authenticated-orcid":true,"given":"Anastasia P.","family":"Litvintseva","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2551-015X","authenticated-orcid":true,"given":"Natalie S.","family":"Nunnally","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay A.","family":"Parnell","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malavika","family":"Rajeev","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia","family":"de Souza Carvalho Melhem","sequence":"additional","affiliation":[{"name":"School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil"},{"name":"Graduate Program in Tropical Diseases, Universidade Estadual Paulista, Botucatu, SP, Brazil"},{"name":"Parasitology and Mycology Center, Instituto Adolfo Lutz, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Possato Fernandes","family":"Takahashi","sequence":"additional","affiliation":[{"name":"School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil"},{"name":"Parasitology and Mycology Center, Instituto Adolfo Lutz, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Manzi","family":"Oliboni","sequence":"additional","affiliation":[{"name":"Graduate Program in Sciences, Coordenadoria de Controle de Doen\u00e7as, Secretary of Health, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Xavier","family":"Bonfieti","sequence":"additional","affiliation":[{"name":"Parasitology and Mycology Center, Instituto Adolfo Lutz, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisandra Siufi","family":"Araujo","sequence":"additional","affiliation":[{"name":"Central Public Health Laboratory of Mato Grosso do Sul, Secretary of Health, Campo Grande, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Cappellano","sequence":"additional","affiliation":[{"name":"Microbiology Section, Grupo Fleury, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Venturini","sequence":"additional","affiliation":[{"name":"School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4383-5994","authenticated-orcid":true,"given":"Shawn R.","family":"Lockhart","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3099-7334","authenticated-orcid":true,"given":"D. Joseph","family":"Sexton","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof8080776"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12760"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2019.100908"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof9020226"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2907.230155"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.2017279"},{"key":"e_1_3_3_8_2","first-page":"507","article-title":"Refractory Sporotrichosis due to Sporothrix Brasiliensis in humans appears to be unrelated to in vivo resistance","volume":"55","author":"Almeida-Paes R","year":"2017","unstructured":"Almeida-Paes R, OliveiraMME, Freitas AFS, Valle ACF, Gutierrez-Galhardo MC, Zancop\u00e9-Oliveira RM. 2017. 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