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Conflicting reports of clearance of<jats:italic>Ehrlichia canis<\/jats:italic>after treatment with doxycycline suggested that the disease phase during which treatment is initiated influences outcomes of these treatments. The purpose of this study was to evaluate the efficacy of a 28-day doxycycline regimen for clearance of experimental<jats:italic>E. canis<\/jats:italic>infections from dogs treated during three phases of the disease. Ten dogs were inoculated with blood from<jats:italic>E. canis<\/jats:italic>carriers and treated with doxycycline during acute, subclinical, or chronic phases of CME. Daily rectal temperatures and semiweekly blood samples were monitored from each dog, and<jats:italic>Rhipicephalus sanguineus<\/jats:italic>ticks were acquisition fed on each dog for xenodiagnosis. Blood collected from dogs treated during acute or subclinical CME became PCR negative for<jats:italic>E. canis<\/jats:italic>as clinical parameters improved, but blood samples collected from dogs treated during chronic CME remained intermittently PCR positive.<jats:italic>R. sanguineus<\/jats:italic>ticks fed on dogs after doxycycline treatments became PCR positive for<jats:italic>E. canis<\/jats:italic>, regardless of when treatment was initiated. However, fewer ticks became PCR positive after feeding on two persistently infected dogs treated with doxycycline followed by rifampin, suggesting that antibiotic therapy can reduce tick acquisition of<jats:italic>E. canis<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/aac.01622-09","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T04:24:45Z","timestamp":1286252685000},"page":"5012-5020","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Efficacy of a Doxycycline Treatment Regimen Initiated during Three Different Phases of Experimental Ehrlichiosis"],"prefix":"10.1128","volume":"54","author":[{"given":"Jennifer C.","family":"McClure","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle L.","family":"Crothers","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Schaefer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick D.","family":"Stanley","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glen R.","family":"Needham","sequence":"additional","affiliation":[{"name":"Department of Entomology, The Ohio State University, Columbus, Ohio 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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