{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:03:43Z","timestamp":1790867023781,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            is the causative agent of several serious infectious diseases. The emergence of antibiotic-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            strains has resulted in significant treatment difficulties, intensifying the need for new antimicrobial agents. Toward this end, we have developed a novel chimeric bacteriophage (phage) lysin that is active against staphylococci, including methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            (MRSA). The chimeric lysin (called ClyS) was obtained by fusing the N-terminal catalytic domain of the\n            <jats:italic>S. aureus<\/jats:italic>\n            Twort phage lysin with the C-terminal cell wall-targeting domain from another\n            <jats:italic>S. aureus<\/jats:italic>\n            phage lysin (phiNM3), which displayed\n            <jats:italic>Staphylococcus<\/jats:italic>\n            -specific binding. ClyS was expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , and the purified protein lysed MRSA, vancomycin-intermediate strains of\n            <jats:italic>S. aureus<\/jats:italic>\n            (VISA), and methicillin-sensitive (MSSA) strains of\n            <jats:italic>S. aureus in vitro<\/jats:italic>\n            . In a mouse nasal decolonization model, a 2-log reduction in the viability of MRSA cells was seen 1 h following a single treatment with ClyS. One intraperitoneal dose of ClyS also protected against death by MRSA in a mouse septicemia model. ClyS showed a typical pattern of synergistic interactions with both vancomycin and oxacillin\n            <jats:italic>in vitro<\/jats:italic>\n            . More importantly, ClyS and oxacillin at doses that were not protective individually protected synergistically against MRSA septic death in a mouse model. These results strongly support the development of ClyS as an attractive addition to the current treatment options of multidrug-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            infections and would allow for the reinstatement of antibiotics shelved because of mounting resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.01625-09","type":"journal-article","created":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T04:53:18Z","timestamp":1263963198000},"page":"1603-1612","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":212,"title":["Synergism between a Novel Chimeric Lysin and Oxacillin Protects against Infection by Methicillin-Resistant\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"54","author":[{"given":"Anu","family":"Daniel","sequence":"first","affiliation":[{"name":"The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., Box 172, New York, New York 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad","family":"Euler","sequence":"additional","affiliation":[{"name":"The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., Box 172, New York, New York 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattias","family":"Collin","sequence":"additional","affiliation":[{"name":"The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., Box 172, New York, New York 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Chahales","sequence":"additional","affiliation":[{"name":"The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., Box 172, New York, New York 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth J.","family":"Gorelick","sequence":"additional","affiliation":[{"name":"The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., Box 172, New York, New York 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent A.","family":"Fischetti","sequence":"additional","affiliation":[{"name":"The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., Box 172, New York, New York 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01933.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700501"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/522355"},{"key":"e_1_3_2_5_2","first-page":"1","volume":"58","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. 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