{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:38:34Z","timestamp":1790516314686,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacter cloacae<\/jats:named-content>\n            isolate was recovered from a rectal swab from a patient hospitalized in France with previous travel to Switzerland. It was resistant to penicillins, narrow- and broad-spectrum cephalosporins, aztreonam, and carbapenems but remained susceptible to expanded-spectrum cephalosporins. Whereas PCR-based identification of the most common carbapenemase genes failed, the biochemical Carba NP test II identified an Ambler class A carbapenemase. Cloning experiments followed by sequencing identified a gene encoding a totally novel class A carbapenemase, FRI-1, sharing 51 to 55% amino acid sequence identity with the closest carbapenemase sequences. However, it shared conserved residues as a source of carbapenemase activity. Purified \u03b2-lactamase FRI-1 hydrolyzed penicillins, aztreonam, and carbapenems but spared expanded-spectrum cephalosporins. The 50% inhibitory concentrations (IC\n            <jats:sub>50<\/jats:sub>\n            s) of clavulanic acid and tazobactam were 10-fold higher than those found for\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            carbapenemase (KPC), IMI, and SME, leading to lower sensitivity of FRI-1 activity to \u03b2-lactamase inhibitors. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>FRI-1<\/jats:sub>\n            gene was located on a ca. 110-kb untypeable, transferable, and non-self-conjugative plasmid. A putative LysR family regulator-encoding gene at the 5\u2032 end of the \u03b2-lactamase gene was identified, leading to inducible expression of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>FRI-1<\/jats:sub>\n            gene.\n          <\/jats:p>","DOI":"10.1128\/aac.01636-15","type":"journal-article","created":{"date-parts":[[2015,9,22]],"date-time":"2015-09-22T02:23:49Z","timestamp":1442888629000},"page":"7420-7425","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Genetic and Biochemical Characterization of FRI-1, a Carbapenem-Hydrolyzing Class A \u03b2-Lactamase from Enterobacter cloacae"],"prefix":"10.1128","volume":"59","author":[{"given":"Laurent","family":"Dortet","sequence":"first","affiliation":[{"name":"Associated National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"Faculty of Medicine, South Paris University, Le 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Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saoussen","family":"Oueslati","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, South Paris University, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Associated National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"Faculty of Medicine, South Paris University, Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Hospital Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, and INSERM U914, Le Kremlin-Bic\u00eatre, France"},{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland"},{"name":"HFR-H\u00f4pital Cantonal, Fribourg, 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