{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T14:52:58Z","timestamp":1774795978750,"version":"3.50.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The clinical strain\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    TO799 was resistant to penicillin-clavulanate combinations and ceftazidime and was not reproducibly detected as an extended-spectrum \u03b2-lactamase (ESBL) according to the standards of the Clinical Laboratory Standards Institute (CLSI; formerly NCCLS) and the national guidelines of the French Society for Microbiology (Comite\u0301 de l'Antibiogramme de la Socie\u0301te\u0301 Franc\u0327aise de Microbiologie). A novel \u03b2-lactamase, designated TEM-125, was responsible for this phenotype. TEM-125 harbors a complex association of mutations previously described in the ESBL TEM-12 and in the inhibitor-resistant \u03b2-lactamase TEM-39. TEM-125 is the first complex mutant TEM to present hydrolytic activity against ceftazidime (\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>cat<\/jats:sub>\n                    , 3.7 s\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) together with a high level of resistance to clavulanate (50% inhibitory concentration, 13.6 \u03bcM). The discovery of such an ESBL, which is difficult to detect by the usual ESBL detection methods, confirms the emergence of a complex mutant TEM subgroup and highlights the need to evaluate detection methods so as to avoid possible therapeutic failures.\n                  <\/jats:p>","DOI":"10.1128\/aac.01639-05","type":"journal-article","created":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T14:26:28Z","timestamp":1151331988000},"page":"2403-2408","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["CMT-Type \u03b2-Lactamase TEM-125, an Emerging Problem for Extended-Spectrum \u03b2-Lactamase Detection"],"prefix":"10.1128","volume":"50","author":[{"given":"Fre\u0301de\u0301ric","family":"Robin","sequence":"first","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie"},{"name":"Universite\u0301 Clermont 1, EA3844, Faculte\u0301 de Me\u0301decine-Pharmacie, Clermont-Ferrand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Delmas","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie"},{"name":"Universite\u0301 Clermont 1, EA3844, Faculte\u0301 de Me\u0301decine-Pharmacie, Clermont-Ferrand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryse","family":"Archambaud","sequence":"additional","affiliation":[{"name":"CHU Toulouse, Ho\u0302pital Rangueil, Laboratoire de Bacte\u0301riologie, Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ce\u0301dric","family":"Schweitzer","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie"},{"name":"Universite\u0301 Clermont 1, EA3844, Faculte\u0301 de Me\u0301decine-Pharmacie, Clermont-Ferrand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Chanal","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie"},{"name":"Universite\u0301 Clermont 1, EA3844, Faculte\u0301 de Me\u0301decine-Pharmacie, Clermont-Ferrand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Bonnet","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bacte\u0301riologie"},{"name":"Universite\u0301 Clermont 1, EA3844, Faculte\u0301 de Me\u0301decine-Pharmacie, Clermont-Ferrand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.969"},{"key":"e_1_3_2_4_2","first-page":"75","volume":"120","year":"1994","unstructured":"Belaaouaj, A., C. Lapoumeroulie, M. M. Canica, G. Vedel, P. Nevot, R. Krishnamoorthy, and G. Paul. 1994. Nucleotide sequences of the genes coding for the TEM-like \u03b2-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2). FEMS Microbiol. Lett.120:75-80.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3061-3068.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.4.447"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.10.2452"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.02.010"},{"key":"e_1_3_2_9_2","unstructured":"Clinical Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing 15th informational supplement (M100-S15). 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