{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:31:22Z","timestamp":1790803882559,"version":"4.1.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Klebsiella pneumoniae<\/jats:named-content>\n carbapenemase (KPC)-producing\n Enterobacteriaceae<\/jats:named-content>\n have emerged as major nosocomial pathogens.\n bla<\/jats:italic>\n KPC<\/jats:sub>\n , commonly located on Tn\n 4401<\/jats:italic>\n , is found in Gram-negative bacterial strains, with the two most common variants,\n bla<\/jats:italic>\n KPC-2<\/jats:sub>\n and\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n , identified in plasmids with diverse genetic backgrounds. In this study, we examined\n bla<\/jats:italic>\n KPC-4<\/jats:sub>\n - and\n bla<\/jats:italic>\n KPC-5<\/jats:sub>\n -bearing plasmids recovered from two\n K. pneumoniae<\/jats:named-content>\n strains, which were isolated from a single New Jersey hospital in 2005 and 2006, respectively. IncN plasmid pBK31551 is 84 kb in length and harbors\n bla<\/jats:italic>\n KPC-4<\/jats:sub>\n ,\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n ,\n qnrB2<\/jats:italic>\n ,\n aac<\/jats:italic>\n (\n 3<\/jats:italic>\n )\n -Ib<\/jats:italic>\n ,\n aph<\/jats:italic>\n (\n 3\u2032<\/jats:italic>\n )\n -I<\/jats:italic>\n ,\n qacF<\/jats:italic>\n ,\n qacE<\/jats:italic>\n \u0394\n 1<\/jats:italic>\n ,\n sul1<\/jats:italic>\n , and\n dfrA14<\/jats:italic>\n , which confer resistance to \u03b2-lactams, quinolones, aminoglycosides, quaternary ammonium compounds, and co-trimoxazole. The conserved regions within pBK31551 are similar to those of other IncN plasmids. Surprisingly, analysis of the Tn\n 4401<\/jats:italic>\n sequence revealed a large IS\n 110<\/jats:italic>\n - and Tn\n 6901<\/jats:italic>\n -carrying element (8.3 kb) inserted into the\n istA<\/jats:italic>\n gene, encoding glyoxalase\/bleomycin resistance, alcohol dehydrogenase, and\n S<\/jats:italic>\n -formylglutathione hydrolase. Plasmid pBK31567 is 47 kb in length and harbors\n bla<\/jats:italic>\n KPC-5<\/jats:sub>\n ,\n dfrA5<\/jats:italic>\n ,\n qacE<\/jats:italic>\n \u0394\n 1<\/jats:italic>\n , and\n sul1<\/jats:italic>\n . pBK31567 belongs to a novel IncX subgroup (IncX5) and possesses a highly syntenic plasmid backbone like other IncX plasmids; however, sequence similarity at the nucleotide level is divergent. The\n bla<\/jats:italic>\n KPC-5<\/jats:sub>\n gene is carried on a Tn\n 4401<\/jats:italic>\n element and differs from the genetic environment of\n bla<\/jats:italic>\n KPC-5<\/jats:sub>\n described in\n Pseudomonas aeruginosa<\/jats:named-content>\n strain P28 from Puerto Rico. This study underscores the genetic diversity of multidrug-resistant plasmids involved in the spread of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n genes and highlights the mobility and plasticity of Tn\n 4401<\/jats:italic>\n . Comparative genomic analysis provides new insights into the evolution and dissemination of KPC plasmids belonging to different incompatibility groups.\n <\/jats:p>","DOI":"10.1128\/aac.01648-12","type":"journal-article","created":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:17:28Z","timestamp":1351729048000},"page":"269-276","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Complete Nucleotide Sequences of\n bla<\/i>\n KPC-4<\/sub>\n - and\n bla<\/i>\n KPC-5<\/sub>\n -Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey"],"prefix":"10.1128","volume":"57","author":[{"given":"Liang","family":"Chen","sequence":"first","affiliation":[{"name":"Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyan D.","family":"Chavda","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry S.","family":"Fraimow","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Cooper University Hospital, Cooper Medical School of Rowan University, Camden, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R.","family":"Mediavilla","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto G.","family":"Melano","sequence":"additional","affiliation":[{"name":"Ontario Agency for Health Protection and Promotion, Toronto, Ontario, Canada"},{"name":"University of Toronto, Toronto, Ontario, Canada"},{"name":"Mount Sinai Hospital, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospitals Case Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA"},{"name":"Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry 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