{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T07:15:14Z","timestamp":1784099714728,"version":"3.55.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Histoplasmosis is a severe infection that affects millions of patients worldwide and is endemic in the Americas. Amphotericin B (AMB) and itraconazole are highly effective for the treatment of severe and milder forms of the disease, but AMB is toxic, and the bioavailability of itraconazole is erratic. Therefore, it is important to investigate new classes of drugs for histoplasmosis treatment. In this study, a series of nine isoniazid hydrazone derivatives were synthesized and evaluated for their antifungal activities\n            <jats:italic>in vitro<\/jats:italic>\n            against the dimorphic fungus\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Histoplasma capsulatum<\/jats:named-content>\n            var.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">capsulatum<\/jats:named-content>\n            . The drugs were tested by microdilution in accordance with CLSI guidelines. The compound\n            <jats:italic>N<\/jats:italic>\n            \u2032-(1-phenylethylidene)isonicotinohydrazide had the lowest MIC range of all the compounds for the yeast and filamentous forms of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. capsulatum<\/jats:named-content>\n            . The\n            <jats:italic>in vitro<\/jats:italic>\n            synergy of this compound with AMB against the planktonic and biofilm forms of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. capsulatum<\/jats:named-content>\n            cells was assessed by the checkerboard method. The effects of this hydrazone on cellular ergosterol content and membrane integrity were also investigated. The study showed that the compound alone is able to reduce the ergosterol content of planktonic cells and can alter the membrane permeability of the fungus. Furthermore, the compound alone or in combination with AMB showed inhibitory effects against mature biofilms of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. capsulatum<\/jats:named-content>\n            .\n            <jats:italic>N<\/jats:italic>\n            \u2032-(1-Phenylethylidene)isonicotinohydrazide alone or combined with AMB might be of interest in the management of histoplasmosis.\n          <\/jats:p>","DOI":"10.1128\/aac.01654-13","type":"journal-article","created":{"date-parts":[[2014,2,11]],"date-time":"2014-02-11T05:12:30Z","timestamp":1392095550000},"page":"2504-2511","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Synthesis and Antifungal Activity\n            <i>In Vitro<\/i>\n            of Isoniazid Derivatives against Histoplasma capsulatum var. capsulatum"],"prefix":"10.1128","volume":"58","author":[{"given":"Rossana","family":"de Aguiar Cordeiro","sequence":"first","affiliation":[{"name":"Postgraduate Program in Medical Microbiology, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"},{"name":"Postgraduate Program in Medical Sciences, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca Jakelyne","family":"de Farias Marques","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medical Microbiology, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"de Aguiar Cordeiro","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Reinaldo","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Organic and Inorganic Chemistry, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Donato Maia Malaquias","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medical Sciences, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlline Vl\u00e1dia","family":"Silva de Melo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medical Microbiology, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jair","family":"Mafezoli","sequence":"additional","affiliation":[{"name":"Department of Organic and Inorganic Chemistry, Federal University of Cear\u00e1, Fortaleza, Cear\u00e1, 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