{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:38:08Z","timestamp":1790516288998,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Extended-spectrum \u03b2-lactamases (ESBLs) of the CTX-M type are increasingly being reported worldwide, with more than 90 known variants. Clinical\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            isolate Bre-1 was isolated in 2009 and displayed an unusual ESBL phenotype, made of a synergy image between expanded cephalosporins and clavulanic acid discs and susceptibility to penicillins.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            Bre-1 harbored a novel CTX-M-encoding gene, designated\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-93<\/jats:sub>\n            . CTX-M-93 differed from CTX-M-27 by only a single L169Q substitution. Compared to CTX-M-27, CTX-M-93 conferred higher MICs of ceftazidime for\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            (MIC of 8 versus 1.5 \u03bcg\/ml) and decreased MICs of other expanded-cephalosporins (MIC of cefotaxime of 1 versus 32 \u03bcg\/ml) and penicillins (MIC of ticarcillin of 0.5 versus &gt;256 \u03bcg\/ml). A comparison of enzymatic properties revealed that the L169Q substitution led to a decreased\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            for ceftazidime (25.5 versus 330 \u03bcM) but decreased hydrolytic activity against good substrates, such as cefotaxime (\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            of 0.6 versus 113 s\n            <jats:sup>\u22121<\/jats:sup>\n            ), probably owing to the alteration of the omega loop positioning during the catalytic process. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-93<\/jats:sub>\n            gene was surrounded by the IS\n            <jats:italic>Ecp1<\/jats:italic>\n            and IS\n            <jats:italic>903<\/jats:italic>\n            elements and inserted onto a 150-kb non-self-transferrable IncF-type plasmid.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            Bre-1 belongs to phylogroup D and is of multilocus sequence type (MLST) 624, a sequence type found only in rare Spanish CTX-M-14-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            isolates. We have characterized a novel CTX-M variant, CTX-M-93, lacking significant penicillin hydrolysis but with increased ceftazidime hydrolysis.\n          <\/jats:p>","DOI":"10.1128\/aac.01656-10","type":"journal-article","created":{"date-parts":[[2011,2,23]],"date-time":"2011-02-23T04:16:50Z","timestamp":1298434610000},"page":"1861-1866","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["CTX-M-93, a CTX-M Variant Lacking Penicillin Hydrolytic Activity"],"prefix":"10.1128","volume":"55","author":[{"given":"Laura","family":"Djamdjian","sequence":"first","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique-H\u00f4pitaux de Paris, and Facult\u00e9 de M\u00e9decine Paris-Sud, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique-H\u00f4pitaux de Paris, and Facult\u00e9 de M\u00e9decine Paris-Sud, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Tand\u00e9","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Microbiologie, Centre Hospitalier Universitaire (CHU) Brest, EA No. 3882-IFR148, Universit\u00e9 de Bretagne Occidentale, Brest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaelle","family":"Cuzon","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique-H\u00f4pitaux de Paris, and Facult\u00e9 de M\u00e9decine Paris-Sud, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Hanrotel-Saliou","sequence":"additional","affiliation":[{"name":"D\u00e9partement de N\u00e9phrologie, Centre Hospitalier Universitaire (CHU) Brest, EA No. 3882-IFR148, Universit\u00e9 de Bretagne Occidentale, Brest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique-H\u00f4pitaux de Paris, and Facult\u00e9 de M\u00e9decine Paris-Sud, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl252"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.8.2269-2275.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg256"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01035-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1037-1044.2005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_2_11_2","unstructured":"Clinical and Laboratory Standards Institute. 2007. 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