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Hereby, it was found, first, that mutations on several charged residues in cofilin to alanine, D19A-R21A, E48A, and K36A, increased the formation of reactive oxygen species and induced apoptosis along with typical hallmarks, including mitochondrial membrane potential depolarization, cytochrome\n            <jats:italic>c<\/jats:italic>\n            release, upregulation of metacaspases, and DNA cleavage, in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . Two of these mutations (D19A-R21A and K36A) increased acetyl coenzyme A and ATP concentrations by triggering fatty acid \u03b2-oxidation. The upregulated acetyl coenzyme A affected the ergosterol biosynthetic pathway, leading to overexpression of\n            <jats:italic>cyp51A<\/jats:italic>\n            and -\n            <jats:italic>B<\/jats:italic>\n            , while excess ATP fueled ATP-binding cassette transporters. Besides, both of these mutations reduced the susceptibility of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            to azole drugs and enhanced the virulence of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            in a\n            <jats:named-content content-type=\"genus-species\">Galleria mellonella<\/jats:named-content>\n            infection model. Taken together, novel and key charged residues in cofilin were identified to be essential modules regulating the mitochondrial function involved in azole susceptibility, apoptosis, and virulence of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.01659-17","type":"journal-article","created":{"date-parts":[[2018,3,28]],"date-time":"2018-03-28T16:15:04Z","timestamp":1522253704000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Identification and Characterization of Key Charged Residues in the Cofilin Protein Involved in Azole Susceptibility, Apoptosis, and Virulence of Aspergillus fumigatus"],"prefix":"10.1128","volume":"62","author":[{"given":"Zhongyi","family":"Lu","sequence":"first","affiliation":[{"name":"Academy of Military Medical Sciences, Academy of Military Sciences, PLA, Beijing, 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