{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:05:24Z","timestamp":1791273924717,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>\n            peptidoglycan is cross-linked mainly by\n            <jats:sc>l<\/jats:sc>\n            ,\n            <jats:sc>d<\/jats:sc>\n            -transpeptidases (LDTs), which are efficiently inactivated by a single \u03b2-lactam class, the carbapenems. Development of carbapenems for tuberculosis treatment has recently raised considerable interest since these drugs, in association with the \u03b2-lactamase inhibitor clavulanic acid, are uniformly active against extensively drug-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. tuberculosis<\/jats:named-content>\n            and kill both exponentially growing and dormant forms of the bacilli. We have purified the five\n            <jats:sc>l<\/jats:sc>\n            ,\n            <jats:sc>d<\/jats:sc>\n            -transpeptidase paralogues of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. tuberculosis<\/jats:named-content>\n            (Mt1 to -5) and compared their activities with those of peptidoglycan fragments and carbapenems. The five LDTs were functional\n            <jats:italic>in vitro<\/jats:italic>\n            since they were active in assays of peptidoglycan cross-linking (Mt5), \u03b2-lactam acylation (Mt3), or both (Mt1, Mt2, and Mt4). Mt3 was the only LDT that was inactive in the cross-linking assay, suggesting that this enzyme might be involved in other cellular functions such as the anchoring of proteins to peptidoglycan, as shown in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            . Inactivation of LDTs by carbapenems is a two-step reaction comprising reversible formation of a tetrahedral intermediate, the oxyanion, followed by irreversible rupture of the \u03b2-lactam ring that leads to formation of a stable acyl enzyme. Determination of the rate constants for these two steps revealed important differences (up to 460-fold) between carbapenems, which affected the velocity of oxyanion and acyl enzyme formation. Imipenem inactivated LDTs more rapidly than ertapenem, and both drugs were more efficient than meropenem and doripenem, indicating that modification of the carbapenem side chain could be used to optimize their antimycobacterial activity.\n          <\/jats:p>","DOI":"10.1128\/aac.01663-13","type":"journal-article","created":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T03:45:54Z","timestamp":1379389554000},"page":"5940-5945","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":169,"title":["<i>In Vitro<\/i>\n            Cross-Linking of Mycobacterium tuberculosis Peptidoglycan by\n            <scp>l<\/scp>\n            ,\n            <scp>d<\/scp>\n            -Transpeptidases and Inactivation of These Enzymes by Carbapenems"],"prefix":"10.1128","volume":"57","author":[{"given":"Mathilde","family":"Cordillot","sequence":"first","affiliation":[{"name":"Centre de Recherche des Cordeliers, LRMA, Equipe 12, Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S 872, Paris, France"},{"name":"INSERM, U872, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, UMR S 872, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Dub\u00e9e","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, LRMA, Equipe 12, Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S 872, Paris, France"},{"name":"INSERM, U872, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, UMR S 872, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Triboulet","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, LRMA, Equipe 12, Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S 872, Paris, France"},{"name":"INSERM, U872, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, UMR S 872, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Dubost","sequence":"additional","affiliation":[{"name":"Mus\u00e9um National d'Histoire Naturelle, USM0502, Plateforme de Spectrom\u00e9trie de Masse et de Prot\u00e9omique du Mus\u00e9um, Paris, France"},{"name":"CNRS, UMR8041, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arul","family":"Marie","sequence":"additional","affiliation":[{"name":"Mus\u00e9um National d'Histoire Naturelle, USM0502, Plateforme de Spectrom\u00e9trie de Masse et de Prot\u00e9omique du Mus\u00e9um, Paris, France"},{"name":"CNRS, UMR8041, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Emmanuel","family":"Hugonnet","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, LRMA, Equipe 12, Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S 872, Paris, France"},{"name":"INSERM, U872, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, UMR S 872, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Arthur","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, LRMA, Equipe 12, Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S 872, Paris, France"},{"name":"INSERM, U872, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, UMR S 872, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Mainardi","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, LRMA, Equipe 12, Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S 872, Paris, France"},{"name":"INSERM, U872, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, UMR S 872, Paris, France"},{"name":"Assistance Publique-H\u00f4pitaux de Paris, H\u00f4pital Europ\u00e9en Georges Pompidou, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"WHO report","author":"Anonymous","year":"2012","unstructured":"Anonymous. 2012. 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