{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T23:59:45Z","timestamp":1781567985034,"version":"3.54.5"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI131939"],"award-info":[{"award-number":["R01AI131939"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,12,16]]},"abstract":"<jats:p>\n            Fluoroquinolones (FQ) are crucial components of multidrug-resistant tuberculosis (MDR TB) treatment. Differing levels of resistance are associated with specific mutations within the\n            <jats:underline>q<\/jats:underline>\n            uinolone-\n            <jats:underline>r<\/jats:underline>\n            esistance-\n            <jats:underline>d<\/jats:underline>\n            etermining\n            <jats:underline>r<\/jats:underline>\n            egion (QRDR) of\n            <jats:italic>gyrA<\/jats:italic>\n            . We sequenced the QRDR from serial isolates of MDR TB patients in the Preserving Effective TB Treatment Study (PETTS) with baseline FQ resistance (FQ\n            <jats:sup>R<\/jats:sup>\n            ) or acquired FQ resistance (FQ\n            <jats:sup>ACQR<\/jats:sup>\n            ) using an Ion Torrent Personal Genome Machine (PGM) to a depth of 10,000\u00d7 and reported single nucleotide polymorphisms in \u22651% of reads.\n          <\/jats:p>","DOI":"10.1128\/aac.01663-20","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T13:27:11Z","timestamp":1603459631000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Evaluation of Fluoroquinolone Resistance in Serial Mycobacterium tuberculosis Isolates from Individuals Diagnosed with Multidrug-Resistant Tuberculosis"],"prefix":"10.1128","volume":"65","author":[{"given":"Melisa","family":"Willby","sequence":"first","affiliation":[{"name":"Division of Tuberculosis Elimination, National Center for HIV\/AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paige","family":"Chopra","sequence":"additional","affiliation":[{"name":"Division of Tuberculosis Elimination, National Center for HIV\/AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darrin","family":"Lemmer","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Klein","sequence":"additional","affiliation":[{"name":"Division of Tuberculosis Elimination, National Center for HIV\/AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy L.","family":"Dalton","sequence":"additional","affiliation":[{"name":"Division of Tuberculosis Elimination, National Center for HIV\/AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5945-059X","authenticated-orcid":false,"given":"David M.","family":"Engelthaler","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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