{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:53:52Z","timestamp":1785419632793,"version":"3.56.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycobacterium abscessus<\/jats:italic>\n                      exhibits high intrinsic drug resistance, requiring combination therapy. We developed a silkworm (\n                      <jats:italic toggle=\"yes\">Bombyx mori<\/jats:italic>\n                      ) infection model as a whole-organism,\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      -based platform for quantitative, outcome-based evaluations of antimicrobial combinations. The system, examined using clarithromycin-amikacin and imipenem-cefoxitin combinations, showed interaction profiles that were qualitatively consistent with those observed\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      . This rapid, reproducible, and ethical assay enables reliable phenotypic assessments of synergistic or antagonistic effects and may facilitate the evaluation and prioritization of antimicrobial combination regimens in preclinical studies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01665-25","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T13:39:28Z","timestamp":1771508368000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of an\n                    <i>in vivo<\/i>\n                    -based assay using a silkworm infection model for phenotypic evaluation of antimicrobial drug combinations against\n                    <i>Mycobacterium abscessus<\/i>"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4845-6791","authenticated-orcid":true,"given":"Akiho","family":"Yagi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0264zxa45","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Natural Product Chemistry, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University","place":["Sendai, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7111-8012","authenticated-orcid":true,"given":"Motoko","family":"Shinohara","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/046f6cx68","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Disease Research, Fujita Health University","place":["Toyoake, 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