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New drugs or new combination therapies to fight these parasites are urgently needed. Venturicidin A, a macrolide extracted from\n              <jats:italic>Streptomyces<\/jats:italic>\n              , inhibits the ATP synthase complex of fungi and bacteria. However, its effect on trypanosomatids is not fully understood. In this study, we tested venturicidin A on a panel of trypanosomatid parasites using Alamar Blue assays and found it to be highly active against\n              <jats:italic>Trypanosoma brucei<\/jats:italic>\n              and\n              <jats:italic>Leishmania donovani<\/jats:italic>\n              , but much less so against\n              <jats:italic>Trypanosoma evansi<\/jats:italic>\n              . Using fluorescence microscopy, we observed a rapid loss of the mitochondrial membrane potential in\n              <jats:italic>T. brucei<\/jats:italic>\n              bloodstream forms upon venturicidin A treatment. Additionally, we report the loss of mitochondrial DNA in approximately 40%\u201350% of the treated parasites. We conclude that venturicidin A targets the ATP synthase of\n              <jats:italic>T. brucei<\/jats:italic>\n              , and we suggest that this macrolide could be a candidate for anti-trypanosomatid drug repurposing, drug combinations, or medicinal chemistry programs.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01671-23","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T13:11:36Z","timestamp":1718284296000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Venturicidin A affects the mitochondrial membrane potential and induces kDNA loss in\n            <i>Trypanosoma brucei<\/i>"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6532-1992","authenticated-orcid":false,"given":"Dennis A.","family":"Hauser","sequence":"first","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Allschwil, 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