{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:46:17Z","timestamp":1785851177127,"version":"3.56.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Cefiderocol (FDC) is a new siderophore-conjugated cephalosporin that enters the periplasm\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      iron transport systems. However, the specific contribution of individual iron uptake pathways to FDC activity remains unclear. We investigated the role of 13 iron acquisition systems using several\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      strain collections. FDC MICs were determined in iron-depleted and iron-supplemented media for\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      mutants (Keio and pathogenic island [PAI]-deleted collections) and for no-acquired \u03b2-lactamase or TEM (NoBL\/TEM-\n                      <jats:italic toggle=\"yes\">Ec<\/jats:italic>\n                      ) and NDM-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      (NDM-\n                      <jats:italic toggle=\"yes\">Ec<\/jats:italic>\n                      ) clinical isolates. Prevalence of iron uptake genes was assessed in these isolates, and\n                      <jats:italic toggle=\"yes\">fec<\/jats:italic>\n                      operon prevalence and genomic location were investigated in\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      genomes from EnteroBase and RefSeq databases. Compared to K-12 and 536 reference\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      , only \u0394\n                      <jats:italic toggle=\"yes\">cirA<\/jats:italic>\n                      and \u0394\n                      <jats:italic toggle=\"yes\">fiu<\/jats:italic>\n                      (enterobactin system) showed increased FDC MICs (8- and 3-fold, respectively), while \u0394\n                      <jats:italic toggle=\"yes\">fecA<\/jats:italic>\n                      and \u0394\n                      <jats:italic toggle=\"yes\">fecB<\/jats:italic>\n                      had lower MICs (3-fold decrease). The\n                      <jats:italic toggle=\"yes\">fec<\/jats:italic>\n                      operon, a known extraintestinal virulence factor, was significantly more prevalent in isolates with FDC MICs above median than below (96% vs. 33% in NoBL\/TEM-\n                      <jats:italic toggle=\"yes\">Ec<\/jats:italic>\n                      ; 100% vs. 0% in NDM-\n                      <jats:italic toggle=\"yes\">Ec<\/jats:italic>\n                      ). According to EUCAST breakpoints, 63.6% of\n                      <jats:italic toggle=\"yes\">fec<\/jats:italic>\n                      -positive NDM-\n                      <jats:italic toggle=\"yes\">Ec<\/jats:italic>\n                      were resistant to FDC, whereas none of the\n                      <jats:italic toggle=\"yes\">fec<\/jats:italic>\n                      -negative were. The\n                      <jats:italic toggle=\"yes\">fec<\/jats:italic>\n                      operon was found in 46.5% of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      genomes, including virulent clones (ST131, 77%) and was mostly chromosome-borne (99%). Plasmid-borne\n                      <jats:italic toggle=\"yes\">fec<\/jats:italic>\n                      closely resembled that of\n                      <jats:italic toggle=\"yes\">Enterobacter hormaechei or Klebsiella pneumoniae<\/jats:italic>\n                      , suggesting interspecies transfer. Our findings highlight the role of Fec in reducing FDC susceptibility and promoting resistance in NDM-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      . They challenge the virulence-resistance trade-off demonstrating the \u201cliaisons dangereuses\u201d between iron and antibiotics.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01679-25","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T14:57:42Z","timestamp":1783695462000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Influence of iron uptake systems on cefiderocol activity in\n                    <i>Escherichia coli<\/i>\n                    : At the crossroads of antibiotic resistance and virulence"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0620-9530","authenticated-orcid":false,"given":"Emilie","family":"Mallecot","sequence":"first","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), H\u00f4pital Bichat-Claude Bernard, Laboratoire de Bact\u00e9riologie","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentine","family":"Berti","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), H\u00f4pital Bichat-Claude Bernard, Laboratoire de Bact\u00e9riologie","place":["Paris, France"]},{"id":[{"id":"https:\/\/ror.org\/02vjkv261","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Sorbonne Paris Nord et Universit\u00e9 Paris Cit\u00e9, INSERM, IAME","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Petitjean","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), H\u00f4pital Bichat-Claude Bernard, Laboratoire de Bact\u00e9riologie","place":["Paris, France"]},{"id":[{"id":"https:\/\/ror.org\/02vjkv261","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Sorbonne Paris Nord et Universit\u00e9 Paris Cit\u00e9, INSERM, IAME","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Meyer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02vjkv261","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Sorbonne Paris Nord et Universit\u00e9 Paris Cit\u00e9, INSERM, IAME","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Signara","family":"Gueye","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), H\u00f4pital Bichat-Claude Bernard, Laboratoire de Bact\u00e9riologie","place":["Paris, France"]},{"id":[{"id":"https:\/\/ror.org\/02vjkv261","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Sorbonne Paris Nord et Universit\u00e9 Paris Cit\u00e9, INSERM, IAME","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9124-2894","authenticated-orcid":false,"given":"Thibaut","family":"Morel-Journel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02vjkv261","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Sorbonne Paris Nord et Universit\u00e9 Paris Cit\u00e9, INSERM, IAME","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3508-4558","authenticated-orcid":true,"given":"Sarah","family":"Timsit","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), H\u00f4pital Bichat-Claude Bernard, Laboratoire de Bact\u00e9riologie","place":["Paris, 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