{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:06:37Z","timestamp":1789563997298,"version":"build-2803163510"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Nine\n aph<\/jats:italic>\n genes, including\n aph(2\u2033)-Ib<\/jats:italic>\n ,\n aph(2\u2033)-Ic<\/jats:italic>\n ,\n aph(2\u2033)-Ig<\/jats:italic>\n ,\n aph(2\u2033)-If<\/jats:italic>\n ,\n aph(2\u2033)-If1<\/jats:italic>\n ,\n aph(2\u2033)-If3<\/jats:italic>\n ,\n aph(2\u2033)-Ih<\/jats:italic>\n ,\n aac(6\u2032)-Ie<\/jats:italic>\n \u2013\n aph(2\u2033)-Ia<\/jats:italic>\n , and\n aac(6\u2032)-Ie<\/jats:italic>\n \u2013\n aph(2\u2033)-If2<\/jats:italic>\n , were previously identified in\n Campylobacter<\/jats:named-content>\n . To measure the contribution of these alleles to aminoglycoside resistance, we cloned nine genes into the pBluescript and expressed them in\n Escherichia coli<\/jats:named-content>\n DH5\u03b1. The nine\n aph<\/jats:italic>\n expressed in\n E. coli<\/jats:named-content>\n showed various levels of resistance to gentamicin, kanamycin, and tobramycin. Three genes,\n aac(6\u2033)-Ie<\/jats:italic>\n \u2013\n aph(2\u2033)-Ia<\/jats:italic>\n ,\n aph2\u2033-If1<\/jats:italic>\n , and\n aph2\u2033-Ig<\/jats:italic>\n , showed increased MICs to amikacin, and five\n aph<\/jats:italic>\n genes were transferrable.\n <\/jats:p>","DOI":"10.1128\/aac.01682-17","type":"journal-article","created":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T16:30:34Z","timestamp":1509985834000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides"],"prefix":"10.1128","volume":"62","author":[{"given":"S.","family":"Zhao","sequence":"first","affiliation":[{"name":"Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. B.","family":"Jones","sequence":"additional","affiliation":[{"name":"Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Young","sequence":"additional","affiliation":[{"name":"Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. F.","family":"McDermott","sequence":"additional","affiliation":[{"name":"Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02049-12"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/313949"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02873-15"},{"key":"e_1_3_2_6_2","volume-title":"National Antimicrobial Resistance Monitoring System retail meat annual report","author":"Food and Drug Administration","year":"2014","unstructured":"Food and Drug Administration. 2014. National Antimicrobial Resistance Monitoring System retail meat annual report U.S. Food and Drug Administration, Washington, DC. https:\/\/www.fda.gov\/AnimalVeterinary\/SafetyHealth\/AntimicrobialResistance\/NationalAntimicrobialResistanceMonitoringSystem\/ucm059103.htm."},{"key":"e_1_3_2_7_2","volume-title":"CLSI supplement M100-S26","author":"CLSI","year":"2016","unstructured":"CLSI. 2016. Performance standards for antimicrobial susceptibility testing, 26th ed. CLSI supplement M100-S26. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"26"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00669-13"},{"key":"e_1_3_2_9_2","volume-title":"National Antimicrobial Resistance Monitoring System retail meat annual report","author":"Food and Drug Administration","year":"2011","unstructured":"Food and Drug Administration. 2011. National Antimicrobial Resistance Monitoring System retail meat annual report. U.S. Food and Drug Administration, Washington, DC."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.6.2454-2459.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00809-12"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01682-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01682-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:12:26Z","timestamp":1645477946000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01682-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1128\/AAC.01682-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.01682-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"2017-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01682-17"}}