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Phage therapy, with its specific bactericidal action, offers a promising solution. However, there remains a shortage of well-characterized ECC-targeting phages, and dosing and timing optimization for ECC-specific phage cocktails is largely unexplored. In this study, we isolated and characterized three novel lytic phages with diverse genome sizes and host ranges. Notably, \u0424EBU8 demonstrated broad-spectrum activity, lysing both\n              <jats:italic>Enterobacter<\/jats:italic>\n              species and\n              <jats:italic>Acinetobacter baumannii<\/jats:italic>\n              . \u0424ECL22 displayed stability across a wide temperature range (4\u201350\u00b0C), pH tolerance (6\u201310), and a burst size of 19 PFU\/cell, with OmpA identified as its receptor. Our formulated phage cocktail, comprising \u0424EBU8, \u0424ECL22, and \u0424ECL30, effectively rescued mice with\n              <jats:italic>E. cloacae<\/jats:italic>\n              bacteremia in a dose-dependent manner, with a mid-dose regimen showing particularly strong efficacy. Immediate phage administration achieved full survival, whereas a combined prophylactic and therapeutic regimen (\u201c\u221224 + 6\u201d) also resulted in 100% survival. These findings highlight the critical roles of dosing and timing in optimizing phage therapy for carbapenem-resistant\n              <jats:italic>Enterobacter<\/jats:italic>\n              infections, with prophylactic use providing a valuable window for delayed treatment and a promising strategy for combating severe bacterial infections.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01683-24","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T14:00:51Z","timestamp":1740578451000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Optimizing phage therapy for carbapenem-resistant\n            <i>Enterobacter cloacae<\/i>\n            bacteremia: insights into dose and 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