{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:55:08Z","timestamp":1791381308596,"version":"4.3.3"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100011879","name":"LabEx LERMIT","doi-asserted-by":"crossref","award":["ANR-10-LABX-33"],"award-info":[{"award-number":["ANR-10-LABX-33"]}],"id":[{"id":"10.13039\/501100011879","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Belgian Ministry of Social Affairs"},{"name":"South Paris University"},{"name":"LabEx IBED"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Horizontal gene transfer may occur between distantly related bacteria, thus leading to genetic plasticity and in some cases to acquisition of novel resistance traits\n            <jats:named-content content-type=\"genus-species\">. Proteus mirabilis<\/jats:named-content>\n            is an enterobacterial species responsible for human infections that may express various acquired \u03b2-lactam resistance genes, including different classes of carbapenemase genes. Here we report a\n            <jats:named-content content-type=\"genus-species\">Proteus mirabilis<\/jats:named-content>\n            clinical isolate (strain 1091) displaying resistance to penicillin, including temocillin, together with reduced susceptibility to carbapenems and susceptibility to expanded-spectrum cephalosporins. Using biochemical tests, significant carbapenem hydrolysis was detected in\n            <jats:named-content content-type=\"genus-species\">P. mirabilis<\/jats:named-content>\n            1091. Since PCR failed to detect acquired carbapenemase genes commonly found in\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            , we used a whole-genome sequencing approach that revealed the presence of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-58<\/jats:sub>\n            class D carbapenemase gene, so far identified only in\n            <jats:named-content content-type=\"genus-species\">Acinetobacter<\/jats:named-content>\n            species. This gene was located on a 3.1-kb element coharboring a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>AmpC<\/jats:sub>\n            -like gene. Remarkably, these two genes were bracketed by putative XerC-XerD binding sites and inserted at a XerC-XerD site located between the terminase-like small- and large-subunit genes of a bacteriophage. Increased expression of the two\n            <jats:italic>bla<\/jats:italic>\n            genes resulted from a 6-time tandem amplification of the element as revealed by Southern blotting. This is the first isolation of a clinical\n            <jats:named-content content-type=\"genus-species\">P. mirabilis<\/jats:named-content>\n            strain producing OXA-58, a class D carbapenemase, and the first description of a XerC-XerD-dependent insertion of antibiotic resistance genes within a bacteriophage. This study revealed a new role for the XerC-XerD recombinase in bacteriophage biology.\n          <\/jats:p>","DOI":"10.1128\/aac.01697-16","type":"journal-article","created":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T02:50:38Z","timestamp":1479178238000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Chromosomal Amplification of the\n            <i>bla<\/i>\n            <sub>OXA-58<\/sub>\n            Carbapenemase Gene in a Proteus mirabilis Clinical Isolate"],"prefix":"10.1128","volume":"61","author":[{"given":"Delphine","family":"Girlich","sequence":"first","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"},{"name":"Ecology and Evolution of Resistance to Antibiotics Unit, Institut Pasteur-APHP-Universit\u00e9 Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2307-3232","authenticated-orcid":false,"given":"R\u00e9my A.","family":"Bonnin","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"},{"name":"Ecology and Evolution of Resistance to Antibiotics Unit, Institut Pasteur-APHP-Universit\u00e9 Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Bogaerts","sequence":"additional","affiliation":[{"name":"Belgian National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, Yvoir, Belgium"},{"name":"CHU UCL Namur, Yvoir, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgane","family":"De Laveleye","sequence":"additional","affiliation":[{"name":"CHU UCL Namur, Yvoir, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel T.","family":"Huang","sequence":"additional","affiliation":[{"name":"Belgian National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, Yvoir, Belgium"},{"name":"CHU UCL Namur, Yvoir, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7384","authenticated-orcid":false,"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"},{"name":"Ecology and Evolution of Resistance to Antibiotics Unit, Institut Pasteur-APHP-Universit\u00e9 Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Glaser","sequence":"additional","affiliation":[{"name":"Ecology and Evolution of Resistance to Antibiotics Unit, Institut Pasteur-APHP-Universit\u00e9 Paris Sud, Paris, France"},{"name":"CNRS UMR3525, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youri","family":"Glupczynski","sequence":"additional","affiliation":[{"name":"Belgian National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, Yvoir, Belgium"},{"name":"CHU UCL Namur, Yvoir, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"},{"name":"Ecology and Evolution of Resistance to Antibiotics Unit, Institut Pasteur-APHP-Universit\u00e9 Paris Sud, Paris, 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