{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:40:36Z","timestamp":1783467636362,"version":"3.55.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Beta-hemolytic\n Streptococcus agalactiae<\/jats:named-content>\n is the leading cause of bacteremia and invasive infections. These diseases are treated with \u03b2-lactams or macrolides, but the emergence of less susceptible and even fully resistant strains is a cause for concern. New bacteriophage lysins could be promising alternatives against such organisms. They hydrolyze the bacterial peptidoglycan at the end of the phage cycle, in order to release the phage progeny. By using a bioinformatic approach to screen several beta-hemolytic streptococci, a gene coding for a lysin was identified on a prophage carried by\n Streptococcus dysgalactiae<\/jats:named-content>\n subsp.\n equisimilis<\/jats:named-content>\n SK1249. The gene product, named PlySK1249, harbored an original three-domain structure with a central cell wall-binding domain surrounded by an N-terminal amidase and a C-terminal CHAP domain. Purified PlySK1249 was highly lytic and bactericidal for\n S. dysgalactiae<\/jats:named-content>\n (2-log\n 10<\/jats:sub>\n CFU\/ml decrease within 15 min). Moreover, it also efficiently killed\n S. agalactiae<\/jats:named-content>\n (1.5-log\n 10<\/jats:sub>\n CFU\/ml decrease within 15 min) but not several streptococcal commensal species. We further investigated the activity of PlySK1249 in a mouse model of\n S. agalactiae<\/jats:named-content>\n bacteremia. Eighty percent of the animals (\n n<\/jats:italic>\n = 10) challenged intraperitoneally with 10\n 6<\/jats:sup>\n CFU of\n S. agalactiae<\/jats:named-content>\n died within 72 h, whereas repeated injections of PlySK1249 (45 mg\/kg 3 times within 24 h) significantly protected the mice (\n P<\/jats:italic>\n < 0.01). Thus, PlySK1249, which was isolated from\n S. dysgalactiae<\/jats:named-content>\n , demonstrated high cross-lytic activity against\n S. agalactiae<\/jats:named-content>\n both\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n . These encouraging results indicated that PlySK1249 might represent a good candidate to be developed as a new enzybiotic for the treatment of systemic\n S. agalactiae<\/jats:named-content>\n infections.\n <\/jats:p>","DOI":"10.1128\/aac.01701-13","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T03:08:58Z","timestamp":1381201738000},"page":"6276-6283","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["In Vitro<\/i>\n Characterization of PlySK1249, a Novel Phage Lysin, and Assessment of Its Antibacterial Activity in a Mouse Model of Streptococcus agalactiae Bacteremia"],"prefix":"10.1128","volume":"57","author":[{"given":"Frank","family":"Oechslin","sequence":"first","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Daraspe","sequence":"additional","affiliation":[{"name":"Electron Microscopy Facility, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlyse","family":"Giddey","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Moreillon","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gr\u00e9gory","family":"Resch","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.57.4.571"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.299.17.2056"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1701-2163(16)30157-8"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/00016340600780508"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-123-6-199509150-00003"},{"key":"e_1_3_3_7_2","first-page":"1","article-title":"Group B streptococcus infection and diabetes: a review","volume":"4","author":"Yanai H","year":"2012","unstructured":"YanaiH HamasakiH TsudaN AdachiH YoshikawaN MoriyamaS MasuiY MishimaS. 2012. 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