{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:14:54Z","timestamp":1790806494622,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Whole-genome sequencing (WGS) was used for the characterization of the frequently extensively drug resistant (XDR)\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>\n            sequence type 175 (ST175) high-risk clone. A total of 18 ST175 isolates recovered from 8 different Spanish hospitals were analyzed; 4 isolates from 4 different French hospitals were included for comparison. The typical resistance profile of ST175 included penicillins, cephalosporins, monobactams, carbapenems, aminoglycosides, and fluoroquinolones. In the phylogenetic analysis, the four French isolates clustered together with two isolates from one of the Spanish regions. Sequence variation was analyzed for 146 chromosomal genes related to antimicrobial resistance, and horizontally acquired genes were explored using online databases. The resistome of ST175 was determined mainly by mutational events; resistance traits common to all or nearly all of the strains included specific\n            <jats:italic>ampR<\/jats:italic>\n            mutations leading to\n            <jats:italic>ampC<\/jats:italic>\n            overexpression, specific mutations in\n            <jats:italic>oprD<\/jats:italic>\n            conferring carbapenem resistance, or a\n            <jats:italic>mexZ<\/jats:italic>\n            mutation leading to MexXY overexpression. All isolates additionally harbored an\n            <jats:italic>aadB<\/jats:italic>\n            gene conferring gentamicin and tobramycin resistance. Several other resistance traits were specific to certain geographic areas, such as a streptomycin resistance gene,\n            <jats:italic>aadA13<\/jats:italic>\n            , detected in all four isolates from France and in the two isolates from the Cantabria region and a\n            <jats:italic>glpT<\/jats:italic>\n            mutation conferring fosfomycin resistance, detected in all but these six isolates. Finally, several unique resistance mutations were detected in single isolates; particularly interesting were those in genes encoding penicillin-binding proteins (PBP1A, PBP3, and PBP4). Thus, these results provide information valuable for understanding the genetic basis of resistance and the dynamics of the dissemination and evolution of high-risk clones.\n          <\/jats:p>","DOI":"10.1128\/aac.01720-16","type":"journal-article","created":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T17:04:42Z","timestamp":1476205482000},"page":"7415-7423","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":120,"title":["Deciphering the Resistome of the Widespread Pseudomonas aeruginosa Sequence Type 175 International High-Risk Clone through Whole-Genome Sequencing"],"prefix":"10.1128","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6452-9945","authenticated-orcid":false,"given":"Gabriel","family":"Cabot","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"L\u00f3pez-Causap\u00e9","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain A.","family":"Ocampo-Sosa","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Marqu\u00e9s de Valdecilla, Instituto de Investigaci\u00f3n Marqu\u00e9s de Valdecilla, Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea M.","family":"Sommer","sequence":"additional","affiliation":[{"name":"Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, H\u00f8rsholm, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda \u00c1ngeles","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda Hospital Bellvitge, Barcelona, Spain, and CIBERES, Instituto de Salud Carlos III, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Zamorano","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Juan","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fe","family":"Tubau","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda Hospital Bellvitge, Barcelona, Spain, and CIBERES, Instituto de Salud Carlos III, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Marqu\u00e9s de Valdecilla, Instituto de Investigaci\u00f3n Marqu\u00e9s de Valdecilla, Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartolom\u00e9","family":"Moy\u00e0","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Son Espases, Instituto de Investigaci\u00f3n Sanitaria de Palma (IdISPa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6673-2883","authenticated-orcid":false,"given":"Carmen","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Servicio de Medicina Interna, Hospital Virgen 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