{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:40:46Z","timestamp":1788050446978,"version":"build-2784847793"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI150649"],"award-info":[{"award-number":["R21AI150649"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Although lung transplant recipients who develop\n              <jats:italic toggle=\"yes\">Ureaplasma-<\/jats:italic>\n              associated hyperammonemia syndrome are treated with\n              <jats:italic toggle=\"yes\">Ureaplasma<\/jats:italic>\n              -targeted antibiotics, optimal therapy is incompletely defined, and typically not based on real-time antimicrobial susceptibility testing results. This study compared levofloxacin, azithromycin, and doxycycline prevention and treatment of\n              <jats:italic toggle=\"yes\">Ureaplasma urealyticum<\/jats:italic>\n              (UU) and\n              <jats:italic toggle=\"yes\">Ureaplasma parvum<\/jats:italic>\n              (UP) infections in an immunosuppressed murine lung infection model. For prophylaxis, mice received antibiotics before infection with UP-FS (susceptible to all study antibiotics), UP-AzmR (azithromycin-resistant), UP-LevR (levofloxacin-resistant), or UU-DoxR (doxycycline-resistant), with lung bacterial loads (color changing units [CCUs]) measured 18 hours following infection. For UP-FS, doxycycline was most active (\u03944.84 log\n              <jats:sub>10<\/jats:sub>\n              CCU\/g;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              = 0.0006), followed by levofloxacin (\u03942.45 log\n              <jats:sub>10<\/jats:sub>\n              CCU\/g;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              = 0.018), with azithromycin yielding a nonsignificant CCU reduction. Doxycycline (\u03941.9 log\n              <jats:sub>10<\/jats:sub>\n              CCU\/g;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              = 0.0025) and levofloxacin (\u03941.3 log\n              <jats:sub>10<\/jats:sub>\n              CCU\/g;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              = 0.004) showed activity against UP-AzmR. Only doxycycline showed activity against UP-LevR (\u03942.28 log\n              <jats:sub>10<\/jats:sub>\n              CCU\/g;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              = 0.0002), and only azithromycin showed activity against UU-DoxR (\u03940.67 log\n              <jats:sub>10<\/jats:sub>\n              CCU\/g;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              = 0.0175). For treatment, antibiotics were administered 24 hours following infection; doxycycline significantly reduced bacterial loads of all study isolates, except UU-DoxR. In addition, azithromycin was active against UP-LevR (\u03941.21 log\n              <jats:sub>10<\/jats:sub>\n              CCU\/g reduction;\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              = 0.0003). In summary, except for doxycycline-resistant UU, doxycycline was active in preventing and treating\n              <jats:italic toggle=\"yes\">Ureaplasma<\/jats:italic>\n              lung infection in immunosuppressed mice.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01724-24","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T13:02:01Z","timestamp":1744635721000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Comparative efficacy of levofloxacin, azithromycin, and doxycycline prophylaxis and treatment in an experimental\n            <i>Ureaplasma<\/i>\n            murine lung infection model"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0054-904X","authenticated-orcid":true,"given":"Derek","family":"Fleming","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic","place":["Rochester, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha Y.","family":"Al-Jabri","sequence":"additional","affiliation":[{"name":"Division of Public Health, Infectious Diseases, and Occupational Medicine, Department of Medicine, Mayo Clinic","place":["Rochester, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-4141","authenticated-orcid":true,"given":"Robin","family":"Patel","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic","place":["Rochester, USA"]},{"name":"Division of Public Health, Infectious Diseases, and Occupational Medicine, Department of Medicine, Mayo Clinic","place":["Rochester, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","unstructured":"Organ Procurement and Transplantation Network (OPTN) and Scientific Registry of Transplant Recipients (SRTR). 2024. 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