{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T23:56:19Z","timestamp":1791676579243,"version":"4.3.4"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An in-house quantitative real-time PCR (qPCR) assay using TaqMan chemistry has been developed to detect NDM-1 carbapenemase genes from bacterial isolates and directly from stool samples. The qPCR amplification of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            DNA was linear over 10 log dilutions (\n            <jats:italic>r<\/jats:italic>\n            <jats:sup>2<\/jats:sup>\n            = 0.99), and the amplification efficiency was 1.03. The qPCR detection limit was reproducibly 1 CFU, or 10 plasmid molecules, and there was no cross-reaction with DNA extracted from several multidrug-resistant bacteria harboring other \u03b2-lactam resistance genes. Feces spiked with decreasing amounts of enterobacterial isolates producing NDM-1 were spread on ChromID ESBL and on CHROMagar KPC media and were subjected to the qPCR. The limits of carbapenem-resistant bacterial detection from stools was reproducibly 1 \u00d7 10\n            <jats:sup>1<\/jats:sup>\n            to 3 \u00d7 10\n            <jats:sup>1<\/jats:sup>\n            CFU\/100 mg feces with ChromID ESBL medium. The CHROMagar KPC culture medium had higher limits of detection (1 \u00d7 10\n            <jats:sup>1<\/jats:sup>\n            to 4 \u00d7 10\n            <jats:sup>3<\/jats:sup>\n            CFU\/ml), especially with bacterial isolates having low carbapenem MICs. The limits of detection with the qPCR assay were reproducibly below 1 \u00d7 10\n            <jats:sup>1<\/jats:sup>\n            CFU\/100 mg of feces by qPCR assay. Samples spiked with NDM-1-negative bacteria were negative by qPCR. The sensitivity and specificity of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            qPCR assay on spiked samples were 100% in both cases. Using an automated DNA extraction system (QIAcube system), the qPCR assay was reproducible. The use of qPCR is likely to shorten the time for\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM-1<\/jats:sub>\n            detection from 48 h to 4 h and will be a valuable tool for outbreak follow-up in order to rapidly isolate colonized patients and assign them to cohorts.\n          <\/jats:p>","DOI":"10.1128\/aac.01734-10","type":"journal-article","created":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T05:21:42Z","timestamp":1308633702000},"page":"4038-4043","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":95,"title":["Real-Time PCR for Detection of NDM-1 Carbapenemase Genes from Spiked Stool Samples"],"prefix":"10.1128","volume":"55","author":[{"given":"Thierry","family":"Naas","sequence":"first","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, 94275 Le Kremlin-Bic\u00eatre, and Assistance Publique-H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayla","family":"Ergani","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, 94275 Le Kremlin-Bic\u00eatre, and Assistance Publique-H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Carr\u00ebr","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, 94275 Le Kremlin-Bic\u00eatre, and Assistance Publique-H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, 94275 Le Kremlin-Bic\u00eatre, and Assistance Publique-H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46909-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01832.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.09.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02277-09"},{"key":"e_1_3_2_6_2","volume-title":"CLSI M100-S20U","author":"Clinical and Laboratory Standards Institute","year":"2010","unstructured":"Clinical and Laboratory Standards Institute. 2010. 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