{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:17:51Z","timestamp":1791908271722,"version":"4.3.4"},"reference-count":44,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:00:00Z","timestamp":1715817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:00:00Z","timestamp":1715817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              This current\n              <jats:italic>in vitro<\/jats:italic>\n              ,\n              <jats:italic>ex vivo<\/jats:italic>\n              , and\n              <jats:italic>in vivo<\/jats:italic>\n              research aims to evaluate and analyze the linalool-zinc oxide nanocomposite (Lin-ZNP) for treating cystic echinococcosis. Lin-ZNP was synthesized using an ethanolic solution of polyvinyl alcohol. The protoscolicidal effects of Lin-ZNP were tested on hydatid cyst protoscoleces (PTS) in both\n              <jats:italic>in vitro<\/jats:italic>\n              and\n              <jats:italic>ex vivo<\/jats:italic>\n              by eosin exclusion test. The study also examined the impact on caspase-3 gene expression and the external structure of PTS. The\n              <jats:italic>in vivo<\/jats:italic>\n              effect was measured by examining hydatid cysts' quantity, dimensions, and weight in mice intraperitoneally infected with 0.5 mL of PTS solution containing 1,000 PTS. The antioxidant and inflammatory cytokine gene expression levels were examined using real-time PCR. Lin-ZNP significantly (\n              <jats:italic>P<\/jats:italic>\n              &lt; 0.001) killed the PTS in both\n              <jats:italic>in vitro<\/jats:italic>\n              and\n              <jats:italic>ex vivo<\/jats:italic>\n              in a dose- and time-dependent manner. The treated PTS exhibited creases and protrusions as a result of bleb formation and upregulation in the gene expression of caspase-3. Upon treatment with Lin-ZNP, there was a significant (\n              <jats:italic>P<\/jats:italic>\n              &lt; 0.001) reduction in the number, diameter, and weight of the hydatid cysts. Treatment with Lin-ZNP nanocomposite led to a significant increase in the expression of antioxidant genes and a notable decrease in oxidative stress markers, and the expression levels of IL-4 and IL-10. Lin-ZNP has the potential to act as a scolicidal agent and demonstrates promise in controlling hydatid cysts in a mouse model, attributed to its antioxidant and anti-inflammatory properties. However, additional studies in clinical trials are needed to confirm the use of Lin-ZNP for treating hydatidosis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01734-23","type":"journal-article","created":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T13:32:23Z","timestamp":1715866343000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["High potency of linalool-zinc oxide nanocomposite as a new agent for cystic echinococcosis treatment"],"prefix":"10.1128","author":[{"given":"Aishah E.","family":"Albalawi","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0254-6424","authenticated-orcid":true,"given":"Abdullah F.","family":"Shater","sequence":"additional","affiliation":[{"name":"Department of Medical 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